Evaluation of beneficial Lactic Acid Bacteria strains as a potential probiotic for the prevention of bovine mastitis

FROLA I.; PELLEGRINO M.; GIRAUDO J.; NADER-MACIAS, MEF; BOGNI C.

Bogotá

Congreso; IMMUNOCOLOMBIA2015 ‐ 11th Congress of the Latin American Association of Immunology ‐ 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología; 2015

Latin American Association of Immunology

The conventional methods for the control of mastitis are basically supported by the adoption of preventive strategies including diagnosis, segregation of the animals, use of improved hygiene and therapeutic protocols, with antibiotics frequently applied. Antibiotics used routinely in dry cow therapies are questioned by the adverse effects in the human health. Probiotics, live microorganisms administered in adequate amounts conferring a health benefit on the host, constitute an alternative tool to bovine mastitis treatment. Probiotics may exert their beneficial effects on the host health by severalmechanisms: adhesion to epithelial cells, colonization, biofilm formation, production of biosurfactants, aggregation and coaggregation, production of antagonistic metabolites (organic acids, hydrogen peroxide, bacteriocins), competition for nutrients, production of enzymes and/or immunomodulation. Lactic acid bacteria (LAB) are the main components of the indigenous microbiota of the teat canal and therefore are optimal candidates to design a speciespecificprobiotic product to prevent mastitis. In the present study, the preventive effect of LAB selected by their beneficial properties: Lactobacillus perolens CRL 1724, Enterococcus hirae CRL 1835 and Lactococcus lactis subsp. lactis CRL 1655, after their infusion by the intramammary route, was investigated. The levels of milk specific antibodies, neutrophil phagocytosis, milk appearance, somatic cell counts (SCC) and LAB recovery in milk were determined, and also the clinical signs of the udder.