Anionic surfaces with minimal charge density can restore burst coagulation of microparticle/exosomedepleted blood plasma

ANGEL CONTRERAS-GARCIA; DESGAGNÉ MAXIME; NOELIA D' ELÍA; CHARLES-HUBERT LAFANTAISIE-FAVREAU; JUAN CARLOS RUIZ; GEORGES-ETIENNE RIVARD; WERTHEIMER MICHEL; PAULA MESSINA; CAROLINE D. HOEMANN

Montreal

Congreso; 10th World Biomaterials Congress; 2016

École Politechnique Montreal

Blood contains microparticles (MP) and exosomes derived from a variety of cell types including activated platelets. MPs are procoagulant because their surfaces can express tissue factor and/or calciumbinding sites for coagulation factors that cooperate to induce burst thrombin activation. Plateletpoor plasma (PPP) contains low levels of MPs and can be induced to coagulate after contact with both hydrophilic and hydrophobic surfaces. In a standard thromboelastography (TEG) assay with plastic cups and pins, PPP undergoes spontaneous burst coagulation, as revealed in the TEG trace by a variable clotting time (10 to 26minutes), a low Angle (~33º) and reproducible low clot tensile strength or Maximum Amplitude (MA ~28 mm). PPP coagulates faster and more reproducibly in TEG cups and pins coated with anionic carboxylaterich nanolayers suggesting FXIIactivation, however the role of MPs is unclear. This study tested the hypothesis that MPs are necessary for PPP burst coagulation in plastic TEG cups, and that anionic surfaces are sufficient to restore burst coagulation of MPdepletedplasma.