INICSA   23916
INSTITUTO DE INVESTIGACIONES EN CIENCIAS DE LA SALUD
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Androgens promote a higher infiltration of neutrophils to the prostate in response to bacterial stimuli
Autor/es:
? SCALERANDI MV, GARCÍA LN, LEIMGRUBER C, PEINETTI N, MALDONADO C Y AA QUINTAR
Reunión:
Congreso; IMMUNOCOLOMBIA 2015 - 11th Congress of the Latin American Association of Immunology; 2015
Resumen:
It has been widely established that androgens have suppressive effects on adaptive immunity. However, there is little evidence about their role on innate immunity and the early inflammatory response. We previously reported that testosterone maintains a reduced expression of key elements for innate immunity and diminishes the antibacterial ability of the prostate. In the present study, we focused on the neutrophils, which are the main cells of innate immune system in clearing bacterial infection. We characterized the neutrophil recruitment to the prostate gland in two complimentary acute models of protatitis under different androgen status. Methodology. Male Wistar rats (3monthold, 250 ? 350 g) were orchiectomized via the scrotal route under ketamine (80 mg∕ kg) ∕ xylazine (8 mg∕ kg) anaesthesia and divided into two groups containing eight animals each. One group received s.c. testosterone (Sustanon, Organon; 2.5 mg/rat/day, group T), while the other was treated with the vehicle (group OX). These groups were further divided into 2 groups (n = 4 per group) and assigned to one of two experimental protocols detailed below. Protocol 1: the animals were anesthetized and subjected to laparotomy to expose the ventral prostate. Bacterial prostatitis (BP) was induced on the day 1 postcastration by direct inoculation of 200 ul of a solution containing uropathogenic E. coli (108 UFC/ml) (OX+BP and T+BP groups). The animals were sacrificed at 5 days after bacterial inoculation and the ventral prostates were harvested and processed for morphological analysis at both photonic and ultrastructural levels. The progression of the infection was assessed by immunocytochemistry by using an antiE. coli antibody, and expression of the antimicrobial peptide βdefensin1. Protocol 2: the animals were inoculated with 50 ul of a solution of lipopolysaccharide (LPS, 20 mg/ml) using the same surgical procedure described above at the day 2 postcastration (OX+LPS and T+LPS groups). At 24 hours after LPS inoculation, the animals were sacrificed and the ventral prostates were harvested and processed for morphological analysis and flow cytometry. To determine to amount of neutrophils infiltrating the gland, the cells obtained after a mechanical and enzymatic digestion of the prostate were incubated with antigranulocyteFITC and antidefensin1 antibodies and analysed with a Cytoron Absolute Flow Cytometer followed by data analysis with the Flowjob software. The values were expressed as mean + standard error of the mean and processed statistically using SPSS 17.0 (SPSS Inc., Chicago, Ill., USA). Student?s t test was used to compare OX+LPS versus T+LPS groups. Significant differences were considered at p