INVESTIGADORES
RAMIREZ Dario
congresos y reuniones científicas
Título:
HYPOTHYROIDISM SENSITIZES ACTIVATION AND NET FORMATION IN NEUTROPHILS
Autor/es:
CORIA, M.; SANDRA E. GOMEZ-MEJIBA; DARIO C. RAMIREZ; MARIA S. GIMENEZ
Lugar:
PUERTO MADRYN, CHUBUT
Reunión:
Congreso; SAIB 2010; 2010
Resumen:
CB-P05
HYPOTHYROIDISM SENSITIZES ACTIVATION AND NET
FORMATION IN NEUTROPHILS
Coria MJ , Gomez-Mejiba SE , Ram¨ªrez DC , Gimenez MS
UNSL, IMIBIO-SL, CONICET. Oklahoma Medical Research
Foundation, OK, USA. E-mail: marie_449@yahoo.com.ar
Upon activation with chemotactic compounds neutrophils or
polymorphonuclear leukocytes (PMN) form extracellular traps
(neutrophil¡¯s extracellular traps, NETs). This event depends on the
activation NADPH oxidase-2 (NOX-2). We think that
hypothyroidism (H) sensitizes activation of PMN. To test this we
used a model of H induced with 6-n-propylthiouracil (PTU) in
female Wistar rats. Rats received drinking water without (control,
CO) or with 0.1 g/L of PTU (hypothyroid, HT) for 30 days. Rats
were euthanized by decapitation and blood was collected for PMN
isolation by gradient density centrifugation. PMN were cultured 24
h in the presence or not of 10 ¦ÌM fMLP. NOX-2, myeloperoxidase
(MPO) and NET formation in PMN, were determined by
immunocytochemistry and confocal imaging in single plan images.
The MPO activity was measured by spectrophotometry using odianisidine
as substrate. Nitric oxide (NO) levels in culture
supernatant were measured as nitrites with the Griess reagent. Our
results show an increased NOX-2 and MPO content in PMN of HT.
Overall MPO activity remained unchanged. Activated PMN from
HT rats showed increased fMLP-induced formation of NETs. Nitrite
production was higher in PMN from HT rats. Our results suggest
that hypothyroidism sensitizes neutrophils to activation and NET
formation. This research will contribute to the understanding the
impact of HT on inflammation.