INVESTIGADORES
COTELLA Evelin Mariel
congresos y reuniones científicas
Título:
Prolonged effects of chronic variable stress during adolescence: implications for vulnerability to emotional disorders
Autor/es:
COTELLA, E. M.; SCARPONI, A; HERMAN, JP; PAGLINI, MG
Lugar:
Mendoza
Reunión:
Workshop; International Workshop of Neuroendocrinology; 2015
Institución organizadora:
Universidad de Mendoza
Resumen:
The adolescent period is known to be highly vulnerable to the effects of adverse environment and it has been demonstrated that adolescent rat males hyper respond to chronic variable stress (CVS). In adults, CVS has been widely used but mainly focused in its immediate effects. Nevertheless, it has been recently shown that the sequelae associated with this type of stress may differ from the immediate effect. We hypothesized that adolescent chronic stress causes life-long impairment in the behavioral and physiological adaptations to stressful experiences. We characterized the behavioral long-term outcomes of the exposure to CVS during adolescence. Materials and methods. Wistar Rats PND40 were subjected to CVS (2 weeks): 5 stressors presented randomly twice daily and twice a week 2 overnight stressors. Controls underwent only normal animal handling. At PND 90, 5 weeks after CVS, animals were evaluated under different paradigms. Fear-related behavior: Rats were exposed to a contextual fear conditioning paradigm (3-min exploration- 3 shocks 1mA, 1s, 1min apart - 3 min). 24 h later, a group was re-exposed to the same context while another group was exposed to a different new context and they were allowed to explore during 5min. Anxiety and depressive-like behavior. Rats were evaluated in the Plus Maze (PM) and the Forced Swim Test (FST). Results: Compared to their controls, adult rats that were subjected to CVS during adolescence explore less when first exposed to a novel environment (p