Early Maternal Separation and Stress during Adulthood: Implications for Glucocorticoid Receptors and Anxiety-like Behavior under Treatment with Tricyclic Antidepressant Amitriptyline

COTELLA, EVELIN M.; DURANDO, PATRICIA E.; SUÁREZ, MARTA M.

Huerta Grande, Pcia. de Córdoba, Argentina

Congreso; XXVII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias; 2011

Sociedad Argentina de Neurociencia

During the development of the Central Nervous System, early environment promotes different pathways of expression of molecules and different neurocircuits in the brain which will determine diverging responses to stress in the adult. This involves two types of nuclear receptors for corticosteroid: mineralocorticoid receptor (MR) and glucocorticoid receptor (GR). They mediate in a coordinate manner the steroid control of HPA activity and behavior. The aim of this work was to measure the immunoreactivity of GR and MR in Hippocampus and the anxiety-like behavior after chronic variable stress (CVS) in rats that were previously early maternally separated (MS). During the CVS protocol in adulthood, animals were treated with antidepressant Amitriptyline (AMI) (10 mg/Kg i.p.). There was a rise in the number of GR positive cells in CA3 in response to CVS combined with MS or with AMI. Regarding MR, CVS evoked a rise in the number of positive cells in CA1 and CA3 (p