INVESTIGADORES
DELGADO Osvaldo Daniel
congresos y reuniones científicas
Título:
PRELIMINARY STUDIES OF NAPHTHALENE-DEGRADING BACTERIA ISOLATED FROM MUELLE STORNI SEDIMENTS
Autor/es:
RIVAS MERCADAL J P; DELGADO O D; DIONISI H; FERRERO M
Lugar:
Bs As. Argentina
Reunión:
Congreso; SAIB 2005; 2005
Resumen:
In order to isolate
naphthalene-degrading bacteria from sediments of a highly contaminated site
with polyaromatic hydrocarbons, three successive enrichment cultures were made
in a synthetic marine media with 0.5 % naphthalene as a sole carbon and energy source.
Growth was performed with agitation in darkness at 25ºC and 15ºC. After
incubation, several colonies were isolated on agar plates at the different
enrichment conditions. DGGE procedure was applied in order to compare the
resulting biodiversity of culturable bacteria and ARDRA method to select
potentially different species of bacteria for further identification.
In a culture-independent
approach, total DNA was purified from the same sediments, and alpha subunits of
initial PAH-dioxygenase genes were amplified using a degenerate primer set,
cloned and sequenced. All analyzed clones in the library were closely related
to phnAc genes from the phenanthrene-degraders Burkholderia sp.
Ch1-1, Ch3-5, Cs1-4 and Eh1-1 (AY367784-7; 99.5 to 99.8% similarity at the
nucleotide level) and Alcaligenes faecalis AFK2 (AB024945;
95.7-95.9% similarity). The presence of these catabolic genes was also search on
the isolated bacteria.