“Study of the mechanism of the antiproliferative action of limonene, isolated from Tilia x viridis, upon a lymphoma cell line: participation of nitric oxide.

MANUELE MG; BARREIRO ARCOS ML; FERRARO G; CREMASCHI GA; ANESINI C.

Buenos Aires

Congreso; XXXIX Reunión Anual de la SAFE; 2007

SAFE

The monoterpenes are non nutricional dietary compounds present in essential oil of vegetable species. In previous studies, we demonstrated that limonene exerts antiproliferative action upon a murine lymphoma cell line (BW 5147). Nitric oxide has been implicated in control of cell proliferation. The aim of the present work was to analyze the participation of nitric oxide in the antiproliferative action of limonene, isolated from Tilia x viridis, analyzing the participation of total nitrites specially NO in cell cycle and apoptosis, by flow cytometry. Results (mean ± SEM): proliferation on tumoral cells dpm (24 hs) Basal 22315 ± 1500; L10ug/ml: 19000 ± 1800; L10ug/ml + L-NAME: 22800 ±1900. Total nitrites 4 hs SI: L10ug/ml: 12.7 ± 1.5; L20 ug/ml: 16 ± 1.2; L 40 ug/ml: 8 ± 0.5. Cell cycle: % of cells in G0/G1 (4 hs): basal: 10.68  ± 1.5; L 10 ug/ml: 23.7 ± 2.0; L20 ug/ml: 20.35 ± 2.2. % of cells in G2/M (4 hs): basal: 32.93 ± 2.5; L10 ug/ml: 23.7 ± 2.0; L10 ug/ml + L-NAME: 34.24 ± 2.5. % of subdiploid cells: basal: 15 ± 1.5; L10ug/ml: 25 ± 2.0; L10 ug/ml + L-NAME: 18 ± 1.2. The limonene presented antiproliferative action on lymphoma cell line, inducing cell arrest in G0/G1 and apoptosis The nitric oxide is an intracellular signal involved in these actions.