INMUNOHISTOCHEMISTRY OF THE INTERSTITIAL CELLS OF CAJAL IN THE ABOMASUM OF CALF

MARQUEZ SG, GALOTTA JM, BARBEITO CG, PORTIANKY EL

Tucumán

Congreso; XXII Congreso de la Sociedad de Biología de Tucumán; 2005

Sociedad de Biología de Tucumán

The interstitial cells of Cajal (ICC) have been described in different mammals. ICC generates and propagates slow waves and plays a critical role in motility regulation in the gastrointestinal tract. The present study analyzed the distribution of c-kit positive cells in the normal abomasum samples obtained six one week old calves. C-kit inmunohistochemistry was performed on 4±1 mm sections from 10% formalin fixed paraffin-embedded specimens, according to the LSAB¨ method of DAKO Co. In myenteric plexus, several types of c-kit positive cells were observed, multipolar periganglionic and intraganglionic positive cells was significantly higher and cell area smaller. This cells present short dendrite processes. In circular muscle tunica, positive cells appeared as bipolar cells with long prolongations, interconnected among them. In longitudinal muscle tunica, positive cells distributed within muscle bundles. The specificity of the reaction anti-c-kit and the location of these cells agree with the descriptions in the stomach of other species. We have identified, for the first time, cells in abomasum with morphological and immunological phenotypes similar to ICC. from 10% formalin fixed paraffin-embedded specimens, according to the LSAB¨ method of DAKO Co. In myenteric plexus, several types of c-kit positive cells were observed, multipolar periganglionic and intraganglionic positive cells was significantly higher and cell area smaller. This cells present short dendrite processes. In circular muscle tunica, positive cells appeared as bipolar cells with long prolongations, interconnected among them. In longitudinal muscle tunica, positive cells distributed within muscle bundles. The specificity of the reaction anti-c-kit and the location of these cells agree with the descriptions in the stomach of other species. We have identified, for the first time, cells in abomasum with morphological and immunological phenotypes similar to ICC. from 10% formalin fixed paraffin-embedded specimens, according to the LSAB¨ method of DAKO Co. In myenteric plexus, several types of c-kit positive cells were observed, multipolar periganglionic and intraganglionic positive cells was significantly higher and cell area smaller. This cells present short dendrite processes. In circular muscle tunica, positive cells appeared as bipolar cells with long prolongations, interconnected among them. In longitudinal muscle tunica, positive cells distributed within muscle bundles. The specificity of the reaction anti-c-kit and the location of these cells agree with the descriptions in the stomach of other species. We have identified, for the first time, cells in abomasum with morphological and immunological phenotypes similar to ICC. ±1 mm sections from 10% formalin fixed paraffin-embedded specimens, according to the LSAB¨ method of DAKO Co. In myenteric plexus, several types of c-kit positive cells were observed, multipolar periganglionic and intraganglionic positive cells was significantly higher and cell area smaller. This cells present short dendrite processes. In circular muscle tunica, positive cells appeared as bipolar cells with long prolongations, interconnected among them. In longitudinal muscle tunica, positive cells distributed within muscle bundles. The specificity of the reaction anti-c-kit and the location of these cells agree with the descriptions in the stomach of other species. We have identified, for the first time, cells in abomasum with morphological and immunological phenotypes similar to ICC.