LECTINHISTOQUÍMICA DE LAS CÉLULAS CALICIFORMES EN EL INTESTINO DEL EQUINO, CERDO Y CONEJO

GALOTTA, J. M.; MÁRQUEZ, S. G.; ZANUZZI, C.; PORTIANSKY, E. L. Y BARBEITO C

Tucumán

Congreso; XXII Jornadas Científicas de la Asociación de Biología de Tucumán; 2005

Asociación de Biología de Tucumán

The carbohydrate expression of goblet cells in the intestine of pigs, rabbits and horses was characterized by a lectinhistochemical study. The intestinal sections were deparaffinized and incubated with the following biotinylated lectins: Con A, WGA, DBA, SBA, PNA, RCA-1 and UEA-I. Except for some rectal goblet cells of pigs and horses, no reactivity was found to Con A. An heterogenous lectin binding pattern of goblet cell was observed in all the intestinal sections of the species studied with DBA, WGA and RCA-1. PNA reactivity was absent in the small intestine, except for some cells in the horse. DBA did not bind to colonic goblet cells in the pig, whereas SBA not only did not label colonic goblet cells of the pig, but also those of the horse. RCA-1 staining was negative in the large intestine of the horse. Most of the goblet cells of the whole intestine were intensely labelled with the lectin UEA-I; however, goblet cells of the small intestine of rabbits, colon of horses and rectum of pigs remained unstained. Our results allowed us to conclude that the lectin binding pattern of the intestinal goblet cells varies among the three analyzed species.