Oxidative damage is the biochemical mechanism of cellular injury in choline deficiency.

REPETTO, MARISA; OSSANI, GEORGINA; CARLE, GERMAN; MONSERRAT, ALBERTO; BOVERIS, ALBERTO

Montevideo, Uruguay

Congreso; V Meeting of SFRBM-South American Group. V International Conference on Peroxynitrite and Reactive Nitrogen Species; 2007

SFRBM-South American Group.

Oxidative damage is the pathogenic mechanism involved in the development of the lesions induced by choline-deficiency (CD). It produces oxidative damage in liver, heart, kidney and brain by lipid peroxidation, decrease of antioxidants, and consumption of tissue alpha-tocopherol. Weanling Wistar male rats were divided into two groups: one group fed a choline-deficient diet (CD); and the other fed a choline-supplemented diet as control (CS). Liver, heart, brain and both kidneys were removed for oxidative damage determinations: thiobarbituric reactive substances (TBARS), and tert-butyl hydroperoxide initiated chemiluminescence (CL-BOOH), and histopathological analysis. The histopathological study showed: hepatic esteatosis (first day), renal necrosis (fifth day), and heart necrosis (seventh day). In tissues homogenates, TBARS increased by 35 % in liver (third day, p