PROTECTION BY DIETARY MENHADEN OIL FROM OXIDATIVE DAMAGE IN RENAL NECROSIS DUE TO CHOLINE DEFICIENCY.

OSSANI, G.; REPETTO, M.; BOVERIS, A.; MONSERRAT, A.

Buenos Aires, Argentina.

Workshop; Oxidative stress and antioxidants; 2009

Facultad de Farmacia y Bioquimica, UBA.

Weanling Wistar male rats fed a choline deficient diet develop acute renal injury, with lesions that vary from tubular to cortical necrosis. In this model fish oil has a protective effect. Weanling Wistar rats were fed ad libitum 4 different diets. 1) a choline deficient diet with corn oil and vegetable hydrogenated oil as source of lipids; 2) the same diet supplemented with choline: 3) a choline deficient diet with menhaden oil (from Brevoortia, s.p.) as a source of lipids; 4) the same diet supplemented with choline. Animals were killed at 2, 4 and 7 days of being fed the experimental diets. The purpose of this experiment was to study if menhaden oil has protective effect from lipid peroxidation and oxidative damage in choline deficiency model. Phospholipids oxidation and homogenate chemiluminescence were evaluated. Histopathologycal study of the kidneys was carried out. Renal necrosis was only observed at the 7th day in rats of diet 1. Creatinine levels were only elevated in rats with renal necrosis. The results indicate that in rats fed a choline deficient diet with vegetable oil, the oxidative damage in kidney homogenates was observed already at the fourth day, but when the choline deficient diet is supplemented with menhaden oil, the oxidative stress was showed at the seventh day. These dataconfirm the protective action of fish oil from oxidative damage.