INVESTIGADORES
REPETTO Marisa Gabriela
congresos y reuniones científicas
Título:
Comparison of oxidative damage induced by high levels of the essential metals copper and iron in rat liver.
Autor/es:
SAPORITO MAGRIÑÁ, CHRISTIAN; MUSACCO SEBIO, ROSARIO; FERRAROTTI, NIDIA; REPETTO, MARISA
Lugar:
San Pablo
Reunión:
Congreso; I Sao Paulo Advanced School on Redox Processes in Biomedicine y VII Meeting of South American Group of the Society of Free Radical Biology and Medicine.; 2011
Institución organizadora:
South American Group of the Society of Free Radical Biology and Medicine.
Resumen:
Copper (Cu) and iron (Fe) are essential biometals necessary for living organisms, however, became toxic at elevated tissue concentrations. Objective: to compare the effects of Cu and Fe in rat liver; to correlate the oxidative damage (OD) with metal liver concentration (LMC); and to estimate the minimum LMC to produce 50% of maximal OD (LMC50%). Methods: Sprague Dowley rats (200 g) received increasing doses of CuSO4 and FeCl2 (0-60 mg/kg, ip) for 16hs. A dose-response curve for each OD parameters was evaluated: in situ-organ (CL) and hydroperoxide (BOOH-CL) chemiluminescence, reactive species to thiobarbituric acid (TBARS); nitric oxide (NO), carbonyl (CO), glutathione ratio (GSH/GSSG), total reactive antioxidant potential (TRAP); activities of NADPHoxidase, superoxide dismutase (SOD), catalase (Cat), glutathione transferase (GT), and glutathione peroxidase (GPx). LMC were determined by atomic spectroscopic absorption. Results: dose showed a positive correlation with LMC (Pearson´s factor, r, p