Comparison of oxidative damage induced by high levels of the essential metals copper and iron in rat liver.

SAPORITO MAGRIÑÁ, CHRISTIAN; MUSACCO SEBIO, ROSARIO; FERRAROTTI, NIDIA; REPETTO, MARISA

San Pablo

Congreso; I Sao Paulo Advanced School on Redox Processes in Biomedicine y VII Meeting of South American Group of the Society of Free Radical Biology and Medicine.; 2011

South American Group of the Society of Free Radical Biology and Medicine.

Copper (Cu) and iron (Fe) are essential biometals necessary for living organisms, however, became toxic at elevated tissue concentrations. Objective: to compare the effects of Cu and Fe in rat liver; to correlate the oxidative damage (OD) with metal liver concentration (LMC); and to estimate the minimum LMC to produce 50% of maximal OD (LMC50%). Methods: Sprague Dowley rats (200 g) received increasing doses of CuSO4 and FeCl2 (0-60 mg/kg, ip) for 16hs. A dose-response curve for each OD parameters was evaluated: in situ-organ (CL) and hydroperoxide (BOOH-CL) chemiluminescence, reactive species to thiobarbituric acid (TBARS); nitric oxide (NO), carbonyl (CO), glutathione ratio (GSH/GSSG), total reactive antioxidant potential (TRAP); activities of NADPHoxidase, superoxide dismutase (SOD), catalase (Cat), glutathione transferase (GT), and glutathione peroxidase (GPx). LMC were determined by atomic spectroscopic absorption. Results: dose showed a positive correlation with LMC (Pearson´s factor, r, p