Evaluation of biocompatible agents on the Botritys cinerea growth parameters

RODRIGUEZ NAVAS, A; RODRIGUEZ ROMERA, M.; QUIROGA, M.I.; COMBINA, M; RIVERO, M.L.; PONSONE, M.L.

Recife

Congreso; V Jornada Sul Americana de Levaduras; 2015

Vitis vinifera L., grape berry, is a non-climacteric fruit with a relatively low rate of physiological activity. Argentina exports table grapes to different countries such as Netherlands, Russia, Brazil and Germany, among others. Red globe table grapes variety are harvested in summer and preserved in a refrigerated storage room (chamber) before exportation. Grape postharvest quality can be affected by a wide range of roots, such as gray root, caused by Botritys cinerea. Maintenance of postharvest quality of table grapes is becoming increasingly significant as the supply of high quality commodities exceeds demands. During grape postharvest period, the pathogen can be controlled by adding SO2, but the emergence of fungicide resistance and increasing consumer demands for reduction in residues on fruit emphasize the need for alternative spoilage control strategies. In this line, the biocompatible agents, whether animal, microbial, vegetable or mineral origin, have a great advantage over chemical fungicides because they have the ability to perform an specific function in living tissues without cause any harm in the host or the environment. The aims of this work was to test the effect of three different biocompatible agents, chitosan (0.5, 1 and 2% w v-1), zeolite (1% w v-1) and two yeasts strains of Metchnicowia pulcherrima RCM2 and Pichia membranefaciens RCM95a (104 and 106 cel mL-1) on the in vitro growth parameters of Botritys cinerea B-24 strain. All the biocompatible agents were individually tested on Potato Dextrose Agar (PDA) and incubate for 7-10 days at 25 ºC. Except for zeolite, all the biocompatible agents showed an increase in the lag phase and diminish in the growth rate (p