Yersinia enterocolitica YopH-deficient strain increases neutrophil recruitment by modulation of CXCR2 expression

DAVE MN; SILVA JE; JERÉZ MB; ARCE ME; FILIPPA VP; ELICABE RJ; GORLINO CV; DI GENARO MS

Buenos Aires

Congreso; II French-Argentine Immunology Congress y LXIII Reunión Anual de la Sociedad Argentina de Inmunología; 2015

SAI

Abstract: Backgorund: YopH is a tyrosine phosphatase critical for virulence of Yersinia enterocolitica (Ye).We have demonstrated that Ye deficient in YopH (Ye ΔyopH) increases neutrophil recruitment in Peyer´s patches (PP). The chemokines CXCL1 and CXCL2 by signaling predominantly through CXC receptor 2 (CXCR2) are crucial for the neutrophil migration. The purpose was to analyze the expression of these chemokines in PP after Ye ΔyopH infection. Methods: C57BL/6 mice were orally infected with Ye ΔyopH or with the wild-type strain Ye WA-314 (1-5 108CFU/mouse). At day 3 after infection, PP were aseptically removed and bacterial counting was performed. CXCL1 was quantified in the homogenates using the CBA Assay and the level of CXCL2 was measured by ELISA. The expression of CXCL1 mRNA was studied by qPCR analysis. The CXCR2 expression as well as neutrophil number in blood and PP were analyzed by flow cytometry. Results. Neutrophil recruitment in PP resulted augmented after Ye ΔyopH infection (p