LECTINHISTOCHEMICAL CHARACTERIZATION OF INTERSTITIAL CELLS OF CAJAL IN CAT INTESTINE

GALOTTA JM, MÁRQUEZ SG, PORTIANSKY EL, BARBEITO C

Potrero de Los Funes San Luis

Otro; XXIV Reunión Científica Anual de la SOCIEDAD DE BIOLOGIA DE CUYO; 2006

SOCIEDAD DE BIOLOGIA DE CUYO

61. LECTINHISTOCHEMICAL CHARACTERIZATION OF INTERSTITIAL CELLS OF CAJAL IN CAT INTESTINE Galotta JM1, Márquez SG1,2, Portiansky EL3, Barbeito C3,4.1, Márquez SG1,2, Portiansky EL3, Barbeito C3,4. 1Anatomía Animal, UCA. 2CBC, UBA. 3Patología General, Veterinaria, UNLP. 4Histología, Veterinaria, UNLP. Veterinaria, UNLP. 4Histología, Veterinaria, UNLP. Anatomía Animal, UCA. 2CBC, UBA. 3Patología General, Veterinaria, UNLP. 4Histología, Veterinaria, UNLP.4Histología, Veterinaria, UNLP. The interstitial cells of Cajal (ICC) are pacemakers and conductors of electrical activity of the gastrointestinal muscles. Using lectinhistochemical techniques, we have characterized the carbohydrate expression of the feline intestine ICC. For this purpose 7 lectins were incubated with paraffin embedded samples of ileum and colon of 3 months old cats. The following lectins were used: Con A (Concanavalia ensiformes), WGA (Triticum vulgare), DBA (Dolichus biflorus), SBA (Glycine max), PNA (Arachis hypogaea), RCA–I (Ricinus communis) and UEA–I (Ulex europeus). The diaminobencidine was used as chromogen. Furthermore, ICCs were localized using polyclonal anti c-kit antibodies in immunohistochemical assays. Con A, WGA and RCA–I lectins stained the ICC localized within the region of the myenteric plexus, clossely associated with ganglia in the small and large intestine. Con A detected oligosaccharides present in the entire cytoplasm while WGA and RCA-I bound exclusively to the plasma membrane. In the colon, Con A also bound to ICC distributed in muscle layers. UEA–I did not bound to ICC. Moreover, DBA was negative in the ileum while PNA and SBA were negative in the colon. We concluded that lectinhistochemical assays using Con A, WGA and RCA-I as lectins can be a complementary study for characterizing the ICC present in the felineintestine.