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89. MORPHOMETRIC STUDY OF PLACENTAS FROM CADMIUM INTOXICATED RATS Díaz MC1, Najle R1, Gómez S1, Vacarezza G1, Quiroga M2, Barbeito CG3.1, Najle R1, Gómez S1, Vacarezza G1, Quiroga M2, Barbeito CG3. 1Dpto. Ciencias Biológicas, 2Dpto. Fisiopatología. Facultad de Ciencias Veterinarias. UNCPBA Campus Universitario. 7000. Tandil. 3Cátedra de Histología y Embriología. Facultad de Ciencias Veterinarias. UNLP. E-mail: maridel@vet.unicen.edu.ar Veterinarias. UNLP. E-mail: maridel@vet.unicen.edu.ar Ciencias Veterinarias. UNCPBA Campus Universitario. 7000. Tandil. 3Cátedra de Histología y Embriología. Facultad de Ciencias Veterinarias. UNLP. E-mail: maridel@vet.unicen.edu.ar Veterinarias. UNLP. E-mail: maridel@vet.unicen.edu.ar Dpto. Ciencias Biológicas, 2Dpto. Fisiopatología. Facultad de Ciencias Veterinarias. UNCPBA Campus Universitario. 7000. Tandil. 3Cátedra de Histología y Embriología. Facultad de Ciencias Veterinarias. UNLP. E-mail: maridel@vet.unicen.edu.ar Veterinarias. UNLP. E-mail: maridel@vet.unicen.edu.ar 3Cátedra de Histología y Embriología. Facultad de Ciencias Veterinarias. UNLP. E-mail: maridel@vet.unicen.edu.ar Exposure to cadmium determines structural changes in the placenta. In the present work, the effect of Cd administered at different pregnancy times (4, 7, 10 and 15 days of gestation) on the placental structure of rat was study. Samples of placentas obtained from Wistar rats, 20 days of pregnancy, both control and administered with CdCl2, 10 mg Cd / kg at 4, 7, 10 and 15 days of gestation. All the placentas were weighed and measured, then processed for paraffin embedding. Histological slices were stained with Hematoxilin/ Eosin, for morphometric analysis. Measures were performed on different fields of each slice determining: a) distance between fetal vessels, b) greater length of fetal vessels and c) maternal space between capillaries. Results were analyzed by ANOVA. Significant statistical differences (P<0.05) were only observed between placental diameters from animals intoxicated on day 4 and controls. In the analyzed placentas, a statistically significant (P<0.05) increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. placental diameters from animals intoxicated on day 4 and controls. In the analyzed placentas, a statistically significant (P<0.05) increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. the placentas were weighed and measured, then processed for paraffin embedding. Histological slices were stained with Hematoxilin/ Eosin, for morphometric analysis. Measures were performed on different fields of each slice determining: a) distance between fetal vessels, b) greater length of fetal vessels and c) maternal space between capillaries. Results were analyzed by ANOVA. Significant statistical differences (P<0.05) were only observed between placental diameters from animals intoxicated on day 4 and controls. In the analyzed placentas, a statistically significant (P<0.05) increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. placental diameters from animals intoxicated on day 4 and controls. In the analyzed placentas, a statistically significant (P<0.05) increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. 2, 10 mg Cd / kg at 4, 7, 10 and 15 days of gestation. All the placentas were weighed and measured, then processed for paraffin embedding. Histological slices were stained with Hematoxilin/ Eosin, for morphometric analysis. Measures were performed on different fields of each slice determining: a) distance between fetal vessels, b) greater length of fetal vessels and c) maternal space between capillaries. Results were analyzed by ANOVA. Significant statistical differences (P<0.05) were only observed between placental diameters from animals intoxicated on day 4 and controls. In the analyzed placentas, a statistically significant (P<0.05) increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. placental diameters from animals intoxicated on day 4 and controls. In the analyzed placentas, a statistically significant (P<0.05) increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. P<0.05) were only observed between placental diameters from animals intoxicated on day 4 and controls. In the analyzed placentas, a statistically significant (P<0.05) increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development. P<0.05) increase in the distance between blood vessels and thickness of maternal space were observed between treated and control animals. Also, a decrease in the length of blood vessels (p<0,05) in treated animals compared to controls was observed. These results show that Cd intoxication affects the placental capillary bed, and could modify the placental blood flow and angiogenesis, which could contribute to a decreased fetal development.

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