LECTINHISTOCHEMICAL STUDY OF RABBIT APPENDIX FOLLICLE ASSOCIATED EPITHELIUM DURING Solanum glaucophyllum INTOXICATION

ZANUZZI CN, FONTANA PA, BARBEITO CG, PORTIANSKY EL, CHINCHILLA LA, GIMENO EJ

Huerta Grande. Córdoba

Congreso; I Reunión Conjunta de Sociedades de Biología de la República Argentina. Huerta Grande. Córdoba. Agosto 2007.; 2007

Sociedades de Biología de la República Argentina

The appendix is one of the rabbit immunocompetent organs. Luminal antigens pass over its follicle associated epithelium (FAE) to initiate local immune responses. Solanum glaucophyllum (Sg) is a calcinogenic plant that contains high levels of 1,25-dihydroxyvitamin D, a hormone that regulates cellular differentiation. We analyzed the effects of Sg on the lectin binding pattern of rabbit appendix’s FAE. Three months-old New Zealand male rabbits received 125 mg/kg Sg leaves powder during 15 or 30 days. Samples were included in paraffin. Three ìm of experimental and control sections were then incubated with the following biotinylated lectins: WGA, CON-A, DBA, SBA, PNA, RCA-1 y UEA-1. We only found differences in the binding pattern of DBA. It moderately bound to the FAE apical glycocalix and M cell intraepithelial pockets in the control group. However, there was a progressive reduction in the labelling of experimental groups’ tissues with time. This might indicate a reduction in N-acetil galactosamine expression, a glucidic residue implicated in microorganism adherence to FAE. were then incubated with the following biotinylated lectins: WGA, CON-A, DBA, SBA, PNA, RCA-1 y UEA-1. We only found differences in the binding pattern of DBA. It moderately bound to the FAE apical glycocalix and M cell intraepithelial pockets in the control group. However, there was a progressive reduction in the labelling of experimental groups’ tissues with time. This might indicate a reduction in N-acetil galactosamine expression, a glucidic residue implicated in microorganism adherence to FAE. calcinogenic plant that contains high levels of 1,25-dihydroxyvitamin D, a hormone that regulates cellular differentiation. We analyzed the effects of Sg on the lectin binding pattern of rabbit appendix’s FAE. Three months-old New Zealand male rabbits received 125 mg/kg Sg leaves powder during 15 or 30 days. Samples were included in paraffin. Three ìm of experimental and control sections were then incubated with the following biotinylated lectins: WGA, CON-A, DBA, SBA, PNA, RCA-1 y UEA-1. We only found differences in the binding pattern of DBA. It moderately bound to the FAE apical glycocalix and M cell intraepithelial pockets in the control group. However, there was a progressive reduction in the labelling of experimental groups’ tissues with time. This might indicate a reduction in N-acetil galactosamine expression, a glucidic residue implicated in microorganism adherence to FAE. were then incubated with the following biotinylated lectins: WGA, CON-A, DBA, SBA, PNA, RCA-1 y UEA-1. We only found differences in the binding pattern of DBA. It moderately bound to the FAE apical glycocalix and M cell intraepithelial pockets in the control group. However, there was a progressive reduction in the labelling of experimental groups’ tissues with time. This might indicate a reduction in N-acetil galactosamine expression, a glucidic residue implicated in microorganism adherence to FAE.