IDENTIFICATION OF THE INTERSTITIAL CELLS OF CAJAL IN THE ABOMASUM OF BOVINES

MáRQUEZ SG, GALOTTA JM, PORTIANKY EL, BARBEITO CG

Huerta Grande. Córdoba.

Congreso; I Reunión Conjunta de Sociedades de Biología de la República Argentina. Huerta Grande. Córdoba. Agosto 2007; 2007

Sociedades de Biología de la República Argentina

The interstitial cells of Cajal (ICC) participate in the control of the gastrointestinal motility. The ICC were described in different mammals including our findings in the digestive organs of bovines, pigs, horses, rabbits and cats. Here we describe the distribution of c-kit positive cells using the CD117 monoclonal antibody. Samples were collected from the pyloric region of abomasums of six healthy cows after slaughter. Tissue samples were fixed and embedded in paraffin. Immunohistochemistry was performed using LSAB® (DAKO) as a detection method. Ten images per slide were captured for morphometric purposes using an image analyser (ImagePro Plus 5.1 - Media Cybernetics). c-kit positive cells were found between muscle layers of the organ either surrounding ganglion cells of myoenteric plexus or isolated or forming clusters of cells. Morphometric parameters of c-kit positive cells reveal an area of 30.95 ± 6.21 ìm2; a major axis of 10.55 ± 1.72 ìm and a minor Axis of 3.87 ± 0.56 a major axis of 10.55 ± 1.72 ìm and a minor Axis of 3.87 ± 0.56 a major axis of 10.55 ± 1.72 ìm and a minor Axis of 3.87 ± 0.56 a major axis of 10.55 ± 1.72 ìm and a minor Axis of 3.87 ± 0.56 ± 6.21 ìm2; a major axis of 10.55 ± 1.72 ìm and a minor Axis of 3.87 ± 0.56± 1.72 ìm and a minor Axis of 3.87 ± 0.56 . Their location in the muscular layers, their relations with ganglion cells of the myoenteric plexus and the immunohistochemical and morphometric characterization led us to identify them as ICC.