C-KIT POSITIVE CELLS IN THE PORCINE STOMACH

GALOTTA JM, MáRQUEZ SG, PORTIANKY EL, BARBEITO CG

Huerta Grande. Córdoba

Congreso; I Reunión Conjunta de Sociedades de Biología de la República Argentina. Huerta Grande. Córdoba. Agosto 2007; 2007

Sociedades de Biología de la República Argentina.

C-kit is a stem cells growth factor receptor. It is expressed in the interstitial cells of Cajal (ICC) of the muscular layer of the gastrointestinal tract. Here we analyze the presence, distribution and morphometric parameters of c-kit positive cells in the stomach of pigs. Samples were obtained from the pyloric portion of the stomach of six months-old healthy pigs at abattoir. Tissues were processed for paraffin embedding. Immunohistochemistry was performed using the anti-c-kit CD 117 monoclonal antibody. LSAB® (DAKO) was applied as a detection method; diaminobencidine was used as a chromogen and haematoxylin was used for counterstaining. Morphometry was performed using digital image analysis (ImagePro Plus 5.1 - Media Cybernetics). Methylene blue staining also revealed the presence of mast cells between the muscle fibres. c-kit positive cells were found in the muscular wall of the stomach, in close contact with neurons of the myoenteric plexus. Cells had a fusiform aspect showing fine prolongations. The morphometric analysis data reported an area of 27.03±7.21 ìm2; major axis: 9.76±2.31 ìm and minor axis: 3.68±0.70 ìm. Immunohistochemistry, anatomical location and morphometric data led us to identify them as ICC. anatomical location and morphometric data led us to identify them as ICC. anatomical location and morphometric data led us to identify them as ICC. 9.76±2.31 ìm and minor axis: 3.68±0.70 ìm. Immunohistochemistry, anatomical location and morphometric data led us to identify them as ICC. anatomical location and morphometric data led us to identify them as ICC. anatomical location and morphometric data led us to identify them as ICC. 9.76±2.31 ìm and minor axis: 3.68±0.70 ìm. Immunohistochemistry, anatomical location and morphometric data led us to identify them as ICC. anatomical location and morphometric data led us to identify them as ICC. anatomical location and morphometric data led us to identify them as ICC. ±7.21 ìm2; major axis: 9.76±2.31 ìm and minor axis: 3.68±0.70 ìm. Immunohistochemistry, anatomical location and morphometric data led us to identify them as ICC. anatomical location and morphometric data led us to identify them as ICC. anatomical location and morphometric data led us to identify them as ICC. ±2.31 ìm and minor axis: 3.68±0.70 ìm. Immunohistochemistry, anatomical location and morphometric data led us to identify them as ICC.