Identification of compounds from Anagallis arvensis L with inhibitory activity against Candida albicans

SOBERÓN, J.R.; PASTORIZA, A.C.; SGARIGLIA, M.A.; CARRASCO JUAREZ, M.B.; JAGER, S.; LABADIE, G.; SAMPIETRO,. D.A.; VATTUONE, M.A.

Congreso; 3rd international conference and exhibition on traditional & alternative medicine; 2015

OMICS

The antifungal activity of Anagallis arvensis L (Primulaceae) extracts was evaluated against Candida albicans (ATCC 10231), alone and in combinatory experiments. Disk diffusion, checkerboard and viable cell count assays were employed to determine the effects of samples and combinations of fluconazole (FLU) + samples against C. albicans. The tincture (i.e. ethanolic extract) exhibited the highest antifungal activity among the assayed samples. This extract was subjected to activity guided isolation experiments, including liquid-liquid extraction and column chromatography on Silica Gel, which yielded an antifugal fraction named G5. G5 showed antifungal activity against C. albicans,with a Minimum Inhibitory Concentration (MIC) = 500 mg of extracted material (EM) . L-1 . The Fractional Inhibitory Concentration (FIC) =0.258 indicated synergistic effect for G5 (125 mg EM . L-1 ) + FLU (0.5 mg L-1) against C. albicans. FLU + G5 combination showed fungicidal effect a against this strain. Four triterpenic saponins derived from oleanolic acid were identified from G5: Anagallisin A, B, C and deglucoanagalloside A. Anagallisin C was the main constituent of G5. Our findings suggest that these compounds would be of interest for further studies dealing with chemical modifications to increase the activity and diminish the fungal resistance to the commercial antifungal drugs.