EXPRESIÓN DE VCAM-1 Y APOJ EN AORTAS DE RATAS DEFICIENTES EN VITAMINA A

GATICA LV; OLIVEROS LB; GIMENEZ MS

Rosario- Santa Fe (Argentina)

Congreso; XLII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB); 2006

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

Vitamin A reduces the degree of lipoperoxidation, regulates the expression of antioxidant enzymes and inhibits atherosclerosis protecting LDL from oxidation. We have previously found increased TBARS levels in serum and aorta, higher NF-kB binding activity and increased iNOS, COX-2 and TNF-alpha expression in aorta of animals fed on a vitamin A-deficient diet. We also communicated disturbances in lipid metabolism in serum and aorta of these animals. In the present work we inform the expression levels of VCAM-1 and Apo J in aorta of vitamin A deficient rats. Wistar male 21 d old rats were fed during three months with free vitamin A diet (-A) and the same diet plus 8 mg of Retinol palmitate/kg of diet (+A). Some -A rats received control diet fifteen days before sacrifice (-A refeeded). Vitamin A deficiency was confirmed in plasma by HPLC. Total RNA was isolated by using TRIzol. The –A group showed increased RNAm expression levels of VCAM-1 and Apo J in aortas compared with +A (p< 0.01 and p< 0.001, respectively) and –A refeeded (p< 0.001 and p< 0.05, respectively). Alterations in redox status and lipid metabolism in aorta could induce changes in the expression levels of VCAM-1 and Apo J in response to the nutritional vitamin A deficiency.