INVESTIGADORES
PEREIRA Claudio Alejandro
congresos y reuniones científicas
Título:
Trypanosoma cruzi putative amino acid transporter genes: A post genomic analysis.
Autor/es:
SILBER, ARIEL; PEREIRA, CA; BOUVIER, LEON; LOPES, CAMILA; TONELLI, RENATA; COLLI, WALTER; ALVES, MARIA JULIA MANSO
Lugar:
Caxambu
Reunión:
Congreso; XIX, Annual Meeting of The Brazilian Society of Protozoology; 2003
Resumen:
The metabolism of Trypanosoma cruzi is largely based on the consumption
of amino acids, mainly proline, and aspartic and glutamic acids, which are the
main carbon and energy sources for several stages of the parasite life cycle. An
arginine kinase that converts arginine in phosphoarginine, a phosphagen with a
role as energy reservoir, was recently described. Also, the literature has established
that Pro, Asp and Glu somehow participate in the T. cruzi differentiation process.
The transport of amino acids into the parasite may be regarded as the first step
of their metabolic pathways, making the identification of genes coding for
transporters a major goal aiming at metabolic research and drug design.
Notwithstanding, the mechanisms by which amino acids are transported into
the cytoplasm in T. cruzi are poorly studied and, surprisingly, no protein with
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
The transport of amino acids into the parasite may be regarded as the first step
of their metabolic pathways, making the identification of genes coding for
transporters a major goal aiming at metabolic research and drug design.
Notwithstanding, the mechanisms by which amino acids are transported into
the cytoplasm in T. cruzi are poorly studied and, surprisingly, no protein with
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
of amino acids, mainly proline, and aspartic and glutamic acids, which are the
main carbon and energy sources for several stages of the parasite life cycle. An
arginine kinase that converts arginine in phosphoarginine, a phosphagen with a
role as energy reservoir, was recently described. Also, the literature has established
that Pro, Asp and Glu somehow participate in the T. cruzi differentiation process.
The transport of amino acids into the parasite may be regarded as the first step
of their metabolic pathways, making the identification of genes coding for
transporters a major goal aiming at metabolic research and drug design.
Notwithstanding, the mechanisms by which amino acids are transported into
the cytoplasm in T. cruzi are poorly studied and, surprisingly, no protein with
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
The transport of amino acids into the parasite may be regarded as the first step
of their metabolic pathways, making the identification of genes coding for
transporters a major goal aiming at metabolic research and drug design.
Notwithstanding, the mechanisms by which amino acids are transported into
the cytoplasm in T. cruzi are poorly studied and, surprisingly, no protein with
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
Trypanosoma cruzi is largely based on the consumption
of amino acids, mainly proline, and aspartic and glutamic acids, which are the
main carbon and energy sources for several stages of the parasite life cycle. An
arginine kinase that converts arginine in phosphoarginine, a phosphagen with a
role as energy reservoir, was recently described. Also, the literature has established
that Pro, Asp and Glu somehow participate in the T. cruzi differentiation process.
The transport of amino acids into the parasite may be regarded as the first step
of their metabolic pathways, making the identification of genes coding for
transporters a major goal aiming at metabolic research and drug design.
Notwithstanding, the mechanisms by which amino acids are transported into
the cytoplasm in T. cruzi are poorly studied and, surprisingly, no protein with
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
The transport of amino acids into the parasite may be regarded as the first step
of their metabolic pathways, making the identification of genes coding for
transporters a major goal aiming at metabolic research and drug design.
Notwithstanding, the mechanisms by which amino acids are transported into
the cytoplasm in T. cruzi are poorly studied and, surprisingly, no protein with
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
T. cruzi differentiation process.
The transport of amino acids into the parasite may be regarded as the first step
of their metabolic pathways, making the identification of genes coding for
transporters a major goal aiming at metabolic research and drug design.
Notwithstanding, the mechanisms by which amino acids are transported into
the cytoplasm in T. cruzi are poorly studied and, surprisingly, no protein with
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
T. cruzi are poorly studied and, surprisingly, no protein with
amino acid transport activity has been characterized, as yet, at the molecular
level. In the present work, advantage has been taken from the information
generated by the T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.
T. cruzi genome project in order to identify genes coding for
putative amino acid transporters. Ten thousand partial sequences corresponding
to ESTs and GSSs have been used to assemble CONTIGs containing 50-100
complete ORFs. The existence of such virtual ORFs was confirmed by PCR and
sequencing, and the fact that they are effectively expressed were indicated by
Northern blot and RT-PCR. Interestingly, some of the obtained CONTIGs
contained more than one ORF coding for putative amino acid transporters. This
observation raises the question whether amino acid transporter genes would be
organized in clusters in the parasite.