Endometrial growth inhibition by natural compounds evaluated as new therapeutic agents for endometriosis

FERELLA L; BASTON JI; BILOTAS M; SINGLA JJ; GONZALEZ A; OLIVARES C; MERESMAN G

Lisboa

Congreso; 31st Annual Meeting European Society of Human Reproduction (ESHRE); 2015

European Society of Human Reproduction (ESHRE)

Study question: The objective of our study was to evaluate the effect of Wogonin (WG), an active constituent of Chinese Herbal Medicine, and two of the main antioxidant compounds found in rosemary extract: Carnosic Acid (CA) and Rosmarinic Acid (RA) on the development of experimental endometriosis both in vitro and in vivo.Summary answer: All natural compounds evaluated exerted an inhibitory effect on endometrial growth or endometriosis development. CA, RA and WG inhibited significantly cell proliferation in human endometrial stromal cell line T-HESC, and in primary cultures. In a murine model, these compounds diminished significantly the development of endometriotic-like lesions, reducing considerably its size.What is known already: Endometriosis is a chronic disease, with high levels of recurrence after standard medical therapy. The past few years have seen a renewed interest in the use of natural compounds due to their advantages: long term administration with minimal side effects. WG is known to inhibit proliferation and induce apoptosis in many kinds of cancerous cells. Similarly, principal components of rosemary (Rosmarinus officinalis) leaves, CA and RA, have been reported to have antioxidant and antitumor activities.Study design, size, duration: Primary cultures of stromal cells from eutopic endometrial biopsies of patients with endometriosis and controls, and the T-HESC cell line were incubated with WG, CA or RA for 24-h. Endometriosis was surgically induced in BALB/c mice. Animals received WG, CA or RA daily from post-surgical day 14 until day 28. Participants/ materials, setting, methods: Cell proliferation was evaluated by MTS assay in primary and T-HESC cell cultures. Mice were randomly assigned to different treatment groups: RA-1mg/kg (n=11); RA-3mg/kg (n=10); CA-2mg/kg (n=11); CA-20mg/kg (n=10); WG-20mg/kg (n=12) and Control (n=8). Animals received CA or RA daily by intraperitoneal injection or WG daily by gastric gavage.Main results and the role of chance: WG 40, 80 and 160 µM; CA 10, 12.5 and 25 µg/ml and RA 25, 50 and 100 µg/ml significantly inhibited cell proliferation in T-HESC cell line (p