APOPTOTIC PROCESSES IN BURSA RELATED TO VACCINATION AGAINST INFECTIOUS BURSAL DISEASE

KILLIAN, MJ.; BOVIEZ, JD.; GAMBAROTTA, MC.; LOMBARDO, DM.

Tandil. Buenos Aires. argentina

Jornada; Primeras Jornadas de Ciencias Morfológicas de las Sierras de Tandil. Universidad del Centro de la Provincia de Buenos Aires (UNCPBA).; 2011

Universidad Nacional del Centro de la Provincia de Buenos Aires.

Infectious bursal disease is a viral disease of young birds that causes apoptosis in the B lymphoid series, leading to immunosuppression. The application of live virus vaccines has been helpful but its apoptotic effects in Fabricious bursa are unknown. This research studied the apoptotic effects of such vaccines on this organ. We worked on 220 one-day broilers, divided into four experimental groups. Two groups were vaccinated with live vaccine strain intermediate soft Lukert and intermediate strain ST-14 respectively. A third group was inoculated with the adjuvant vaccines. The fourth control group received no treatment. Animals were sacrificed in groups to 3-7-14-21-28 days post inoculation. Bursas of Fabricius were analyzed by staining with hematoxylin and eosin, and TUNEL technique for the quantification of apoptotic cells and degree of bursal damage. Data was analyzed by analysis of variance for p