GnRH AND GnRHR PRODUCING CELLS IN PORCINE AND BOVINE OVARIES

GAUNA AÑASCO, LG.; SOÑEZ, MC.; GAZZANEO, P.; LOMBARDO, DM.

Buenos Aires

Jornada; Primer Encuentro Rioplatense De Biologia - XIV Jornadas Anuales De La Sociedad Argentina De Biología. Fundación Pablo Cassará,; 2012

SOCIEDAD ARGENTINA DE BIOLOGÍA. SOCIEDAD URUGUAYA DE BIOCIENCIAS

The local production of GnRH and GnRHR-I have been determined in ovaries of some species of mammalian. The object of this work was the identification of the cells that synthesized GnRH and GnRHR in porcine and bovine ovaries. 10 samples of ovaries from slaughterers, with or without corpus luteum, were fixed in 10% buffered formalin and conventionally histological processed. Immunocytochemistry indirect method (LSAB2® System, Dako) was applied on serial sections of 5 μm, using polyclonal rabbit anti- LHRH 1:2000 and monoclonal anti-hGnRHR 1:100 (Thermo Scientific) after hot antigen retrieval. Rat hypothalamus and hypophysis were employed as positive controls while the negative ones were omitting the primary antibodies. GnRH positive cells were scarce and isolated in both ovaries. They were localized in: internal theca of medium follicles (5-8 mm), granulosa cells of some atretic follicles, rete ovarii, interstitial interfollicular tissue and sometimes in medullar cells. GnRHR was different location in both species. Bovine ovaries: superficial epithelium, ovocytes of preantral and medium follicles, granulose cells of medium follicles, luteal cells. Porcine ovaries: ovocyte of pre-antral and small antral follicles (< 5 mm), scarce granulose cells in medium follicle. The cells which synthesized GnRH and GnRHR were different location in the ovaries of both species. The relationships between animal condition and expression of these molecules must be determined