Participación del AhR en la Apoptosis de las Células de Granulosa Porcina

GUARAGNA, C; LOMBARDO DM.; BARAÑAO, LJ.; PECCI, A

Sociedad Argentina de Biología. IByME. Buenos Aires, Argentina

Jornada; Séptimas Jornadas de la Sociedad Argentina de Biología; 2005

APOPTOSIS OF PORCINE GRANULOSA CELLS ISMEDIATED BY ARYL HYDROCARBON RECEPTORGuaragna C1, Lombardo D1, Bara–ao L2,3, Pecci A3,4.1Fac.Cs. Veterinarias UBA; 2IbyME-CONICET; 3Dpto. Qu’micaBiol—gica, FCEyN-UBA; 4IFIByNE-CONICET.E-mail: carinaguar@fvet.uba.arThe arylhydrocarbon receptor (AhR) is known to mediate toxicresponse to dioxin like TCDD and polycyclic aromatic compoundsd has been extensively characterized from a toxicological viewpoint.However, it has recently been reported that the AhR mayhave a central role in ovarian physiology. The expression of AhRhas been described in the ovary of rat, mouse, rabbit, cow and human.Recent findings indicate that the AhR may have a central rolein regulating follicular growth during pre- and postnatal life. Inbovine, the AhR may be involved in the oocytes maturation promotingthe resumption of meiosis. On the other hand, it is knownthat the follicular atresia begins with the programmed cell death ofgranulosa cells. The purpose of the current study was to explorethe role of the AhR in the apoptosis of porcine granulosa cells(PGC). Granulosa cells obtained by aspiration of 3 to 6 mm porcinefollicles were culture and incubated with the AhR agonist b-naphthoflavone (bNF) and the antagonist a-naphthoflavone (aNF).The apoptosis was examined by annexin V-FITC staining and activityof caspase 3. The results showed that aNF 10 mM inducesPGC apoptosis while bNF 10-20 mM showed no significant changesrespect to the control. The aNF induction was significatively revertedby bNF when PGC were coincubated with the agonist andantagonist simultaneously. The results indicate that AhR may beinvolved in the apoptosis regulation of porcine granulosa cells.