INVESTIGADORES
LOMBARDO Daniel Marcelo
congresos y reuniones científicas
Título:
Localización de Receptores de Neurotrofinas de alta afinidad (proteínas Trks) en el timo de Sus scrofa domesticus. Segunda parte.
Autor/es:
GAUNA AÑASCO LG,; DU SANTOS, MJ.; WIGDOROVITZ, A; LOMBARDO, DM; VEGA, JA; VON LAWZEWITSCH, I.
Lugar:
Tandil, Pcia de Buenos Aire
Reunión:
Congreso; X Congreso Argentino de Ciencias Morfológicas; 2006
Resumen:
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Sus scrofs dom.sticus THYMUS HIGH AFFINITY PROTEINS (TRKS) NEUROTROPHINS RECEPTORS LOCALIZATION. SECOND PART.
Gauna A.asco LG1, Du Santos MJ2, Wigdorovitz A2, Lombardo D1, Vega JA3, Von Lawzewitsch I1. 1Facultad de Ciencias Veterinarias. Universidad de Buenos Aires. E-mail: gauna@fvet.uba.ar; 2Instituo de Virolog.a. INTA. Castelar. Pcia de Buenos Aires; 3Universidad de San Pablo. Madrid. España.
Neurotrophins are known as growing factors that participate in some neuronal populations diferentiation, development and maintenance. On the other hand there are evidences that these molecules can work on other tissues that express mRNA and/ or their receptors, as rat and mouse lymphatic systems and man´s palatine tonsils. This goal of this investigation is to determine Trk receptors localization by means of inmunocitochemistry and western-blot in pig´s thymus. Young pigs thymus (n=20) were immediately fixed in 10% buffered formaldehyde and processed by rutine techniques (H-E) and blue metilene. Trks proteins were labeled with rabbit polyclonal antibodies, with ABC method. In order to detect Trk expression by Western blot, homogenates were electrophoresed in SDS-PAGE 12.5% and developed with Trk specific polyclonal antibodies. TrkA y Citokeratine is positive in medullar reticular epithelial cells. TrkB was visualized in medullar monocyte- macrophage cells series. S100 agrees with reticular cortical cells and TrkC with interdigital dendrytic cell in cortex-medullar union. The three Trk proteins were identified in pig thymus by Western blot analysis. Pig´s thymus Trks proteins develop an apropiate microclimate for tymocytes maturation and differentiation and they also may act in a paracrine way.
