INVESTIGADORES
GONZALEZ MAGLIO Daniel Horacio
congresos y reuniones científicas
Título:
Anti-Ro/SS-A autoantibody detection in Lupus Erythematosus and Sjögren's Syndrome. Their relationship with cutaneous photosensitivity
Autor/es:
MARIELA PAZ; DANIEL GONZÁLEZ MAGLIO; MARÍA PINO; ALEJANDRA PELUZZONI; PATRICIA TROIELLI; GUSTAVO NASSWETTER; JULIANA LEONI
Lugar:
Córdoba, Argentina.
Reunión:
Congreso; VII Latin American Congress of Immunology; 2005
Institución organizadora:
Asociación Latinoamericana de Inmunología
Resumen:
Objectives To evaluate the prevalence of anti-Ro/SS-A antibodies in Lupus Erythematosus
(LE) and Sjögrens Syndrome (SS) patients using two ELISA methods, described as
having different sensitivity.
To verify if there is any association
between anti-Ro/SS-A antibodies and the cutaneous photosensitivity
manifestation, since there are many discrepancies about it, although literature
suggests there is a positive correlation.
Methods
Sera from 84 patients were studied by an
ELISA (#1), which uses recombinant
proteins to detect anti-Ro/SS-A 52 and 60 kDa independently. These results were
contrasted with the obtained by a commercial ELISA (#2 INOVA Diagnostics) where total anti-Ro/SS-A antibodies are
detected.
Results
ELISA #1: 33% positive patients (21% LE and 4% SS). ELISA #2: 44% positive patients (26% LE
and 7% SS).
63% of the patients
had photosensitivity; 52% of photosensitive patients were anti-Ro/SS-A negative
by ELISA #2 (64% LE 43% without pathology).
Conclusion
The contrast between the techniques
mentioned indicates that the detection using Ro/SS-A 52 and 60 kDa proteins
together is more sensitive than the method which uses them separately.
It was confirmed that there is not a
direct association between anti-Ro/SS-A antibodies and photosensitivity, at
least in the studied population.