INVESTIGADORES
SOSA ESCUDERO Miguel Angel
congresos y reuniones científicas
Título:
Abietane obtained from salvia cuspidata is active against t.cruzi. XXVII Reunión de la Sociedad Argentina de Protozoología (SAP).
Autor/es:
SPINA R; LOZANO E; SOSA MA; TONN C; CIFUENTES D
Reunión:
Congreso; XXVII Reunión de la Sociedad Argentina de Protozoología (SAP); 2014
Institución organizadora:
Sociedad Argentina de Protozoología (SAP)
Resumen:
T.cruzi is a monoflagellate parasite, responsible
for the Chagas` disease, that affects millions of people in Latinamerica. Since
decades, many natural and synthetic compounds have been tested, but only
Benznidazole and Nifurtimox remain as therapeutic agents against this disease. However, their use is restricted because of
the toxic and side effects. Therefore, the search for new molecules with trypanocidal
activity and with low toxicity to the host cells is required. Terpenoid and
derivatives are attractive because they are abundant in the plant Kingdom and
many of them showed activity against various parasites.
In this study, we tested an abietane (12-hydroxy-11,14-diketo-6,8,12-abietatrien-19,20-olide)
obtained from Salvia cuspidata (Ruiz
& Pav. Subsp. gilliesii (Benth) J.R.I. Wood ,Lamiaceae) on the in-vitro growth of T.cruzi (strain Dm28C) and evaluated the effects on ultrastructure of
parasites. Parasites (epimastigotes)
were grown for 24-48 h in the Diamond liquid medium, in the presence or the
absence of the compound at different concentrations. The compound showed to be
active against T.cruzi, inhibiting
the growth of parasites at low concentrations (1-10 ug/ml). However, the
compound induced low mortality on parasites at concentrations lesser than 5
ug/ml. In turn, the compound was non toxic to mammalian cells at the
concentrations used. We also observed
that abietane induced an intense vacuolization on parasites, even at 7,5 ug/ml.
Further studies should be done in future
to identify the molecular targets on the parasites and the active groups on the
molecule, through chemical modifications. These promising results are a
breakthrough in the search for new molecules that could be used in future as a
therapeutic agent for the Chagas` disease.