Garlic virus B (GarV-B) AND Garlic virus X (GarV-X): THE SAME VIRUS

MARCOS GIOVANI CELLI; MARIA CECILIA PEROTTO; ADA KARINA TORRICO; VILMA CECILIA CONCI

Nigde

Simposio; 7th International Symposium on Edible Alliaceae; 2015

ISHS - International Society for Horticultural Science

Garlic is infected by a complex of viruses composed Potyvirus, Carlavirus and Allexivirus. The aim of this work was to identify and characterize new viruses infecting garlic in Argentina. In 2013 samples were analyzed garlic (cultivar Mesidrome) by immunosorbent electronmicroscopy and decoration with antisera pull: Onion yellow dwarf virus, Leek yellow stripe virus, Garlic common latent virus, Shallot latent virus, Garlic virus C and Garlic virus A. Were observed a decorated viral particles and other undecorated. Total RNA were extracted and sent for sequencing (Illumina HiSeq 1500). The contigs were compared by Blastn with the GenBank database and showed the presence of a contig (8336 nucleotides, nt) with 98% identity (59% coverage) with the longest sequence GarV-B known to date (JN019813, 4923nt). The identity of nt and amino acid (aa) of the gene encoding the coat protein (CP) with others GarV-B isolates was calculated using Lasergene 8.0.2 software package (DNASTAR, Inc., Madison, WI, USA) and showed higher values of 88.4% and 94.7%, respectively. According to the International Committee on Taxonomy of Viruses (ICTV) criteria for differentiation of Allexivirus species (more than about 72% nt identity or 80% aa identity between their CP or polymerase gene are the same species), the contig was considered the first complete sequence of GarV-B (NC_025789/KM379144). The identity (nt and aa) of the polymerase and CP available sequences of GarV-B with the others Allexivirus published in GenBank were calculated. The identity found was higher than those established by the ICTV in all comparisons (polymerase and CP) among isolates GarV-B and GarV-X. These results suggest that GarV-B and GarV-X are the same viruses.