Phenotypic analysis of Arabidopsis thaliana lines with modified levels of NADP-malic enzymes

ARIAS, C. L.; GERRARD WHEELER, M. C.; SAIGO, M.; DRINCOVICH, M. F.; ANDREO, C. S.

Rosario

Congreso; L Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularFisiología Vegetal; 2014

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Throughout recent years multiple roles have been proposed for the NADP-malic enzymes (ME) of C3 plants at different stages of plant development. In order to reveal physiological processes most influenced by the activity of these enzymes in Arabidopsis thaliana?s metabolism, eight transgenic lines with constitutive alterations in the content of malic enzyme were characterized phenotypically along the whole plant development. Each line showed specific pattern of variations, however, the Arabidopsis line knockout in the cytosolic constitutive isoform ME2 was the most affected. This line presented altered features such as germination, root growth, leaf production, shoot growth and seed production. On the other hand, considering the different stages of the plant life, the reproductive stage was the most affected by changes in ME levels.