LECTINHISTOCHEMICAL PATTERN OF THE UTERUS IN PREGNANT AND NON PREGNANT PLAIN VISCACHA (LAGOSTMUS MAXIMUS)

FLAMINI MA, BARBEITO CG, PORTIANSKY EL

Mar del Plata

Congreso; VI Latin American Symposium on Maternal-Fetal Interaction and Placenta and V Latin American Symposium on Reproductive Immunology Meeting 2015; 2015

Latin American Society on Maternal-Fetal Interaction and Placenta

Objectives: To describe the pattern of glycoconjugates (GCs) in the uterusof pregnant and non-pregnant viscacha using lectinhistochemistry. Theinterest in this species is due its high physiological embryonic death,which could be explained by the above mentioned pattern.Methods: The uteri of wild females in anestrus (A), follicular phase (FP),with early pregnancy (EP) and term pregnancy (PAT) were embedded inparaffin, sectioned and incubated with biotinylated lectins: WGA, CON-A,SBA, DBA, PNA, UEA-1 and RCA-1. LSAB was used as a detection system,diaminobenzidine as the chromogen and hematoxylin as contrast. Positiveand negative controls were used. Glycocalyx, surface epithelium andendometrial glands were evaluated. The labeling intensity was semiquantitativelyestablished: 0: negative, 1: mild, 2: moderate and 3:severe.Results: The lectins WGA, SBA and RCA possessed intense reaction in theglycocalyx of the surface epithelium at all reproductive stages. The lectinUEA-1 was moderately to intensely expressed in females in A and FP, butnegative in pregnant animals. The lectins CON-A and PNA moderatelyreacted in the glycocalyx of females in A and not in pregnant animals.Conclusions: The increase or decrease in the expression of some GCs inthe uterine epithelium during pregnancy may indicate the importance ofthese residues for implantation and placentation.