MESENCHYMAL STEM CELLS CULTURED WITH SYNTHETIC AND/OR NATURAL ESTROGENS AS MODULATING FACTORS OF OSTEOGENESIS IN THERAPEUTIC PROTOCOLS

RODRIGUEZ HA; CLAROS, S; JIMÉNEZ-PALOMO, P; RAMOS JG; LUQUE EH; BECERRA, J; ANDRADES, JA

Torremolinos

Congreso; XIV Congreso de la Sociedad Española de Biología Celular; 2011

Sociedad Española de Biología Celular

MESENCHYMAL STEM CELLS CULTURED WITH SYNTHETIC AND/OR NATURAL ESTROGENS AS MODULATING FACTORS OF OSTEOGENESIS IN THERAPEUTIC PROTOCOLS Rodríguez HA1,2, Claros S1,4, Jiménez-Palomo P1,4, Ramos JG3, Luque EH2, Becerra J1,4, Andrades JA1. horacioarodriguez@hotmail.com 1Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, Campus de Teatinos, University of Málaga, Málaga, España. 2Laboratory of Endocrinology and Hormone-dependent tumors (LETH), Faculty of Biochemistry and Biological Sciences, University City, National University of Litoral, Santa Fe, Argentina. 3Department of Clinical and Quantitative Biochemistry, Faculty of Biochemistry and Biological Sciences, University City, National University of Litoral, Santa Fe, Argentina. 4: Laboratory of Bioengineering and Tissue Regeneration (LABRET-UMA), Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine Bone marrow mesenchymal stem cells (MSCs) are considered a promising cell resource for regenerative medicine and tissue engineering. However, the proportion of MSCs in bone marrow is very low. Although in vitro proliferation can amplify the cell population, excessive duplication can significantly reduce differentiation potentials and capability of tissue formation. Because of effects of estrogen on skeletal homeostasis and osteoblasts/osteoclasts activity, we hypothesized that an estrogen supplement could effectively improve the osteogenic differentiation potentials of bone marrow MSCs in vitro. To this end, MSCs obtained from bone marrow of 12 wk-Fischer 344/IcoCrl rats were exposed to conventional induction media to be differentiated into osteogenic lineage. The differentiation potentials in terms of the specific biomarkers were compared with those of cells exposed to a differentiation medium supplemented with different doses of 17-β-estradiol. Preliminary, our data demonstrate that 17-β-estradiol can modulate the differentiation, and potentially improve the efficiency of MSCs in stem cell–based tissue engineering and regeneration. 17-β-estradiol could be used to reduce the amount of osteoinductive agents required to add to the culture medium. Further study is needed to verify the regulatory functions of estrogens on MSCs differentiations of both human donors and animal models of specific pathological conditions such as osteoporosis. Funding agencies: Ministerio de Ciencia e Innovación (BIO2009-13903-C02-01, Instituto de Salud Carlos III FIS PI10-02529 and Red de Terapia CelularRD06/0010/0014); Junta de Andalucía(P07-CVI-2781, PAIDI, BIO-217, and Consejería de Salud,PI-0729-2010); Consejo Nacional de investigaciones Científicas y Técnicas(CONICET), Argentina.