INVESTIGADORES
OTERO-LOSADA Matilde Estela
congresos y reuniones científicas
Título:
Insulin resistance and relative beta-cell volume in sweet carbonated beverage-induced metabolic syndrome in the rat
Autor/es:
OTERO-LOSADA M.; GONZALEZ J; MULLER A; OTTAVIANO G; AMBROSIO G.; MILEI J.
Lugar:
Athens
Reunión:
Congreso; EUROPEAN SOCIETY OF HYPERTENSION (ESH) AND INTERNATIONAL SOCIETY OF HYPERTENSION (ISH) JOINT MEETING.; 2014
Institución organizadora:
Eur Society of Hypertension
Resumen:
Purpose: to evaluate
insulin resistance, a key factor in metabolic syndrome pathophysilogy, and the
relative beta-cell volume after 6 months of respectively sucrose-sweet or
sucrose-free beverage drinking and possible reversal of the eventual changes in
rats.
Materials and methods: 48 male Wistar
rats were randomly assigned to 3 groups and were respectively allowed to drink
regular cola (C) (sucrose sweetened carbonated drink), light cola (L)
(low-calorie artificially sweetened carbonated drink) or tap water (W) ad
libitum for 6 months. Determinations were performed at 6 months (?Treatment?)
and 12 months (?Wash-out?, at the end of an additional 6 months´ tap water
drinking period). Glycemia and insulinemia were measured by conventional enzymatic and immunoassay tests
and were used to calculate the HOMA-IR index. At autopsy, pancreatic tissue was
processed and immunostained for insulin (monoclonal anti-insulin antibody). The
relative beta-cell volume (BCV) was quantified on a software-basis. Data were analyzed
by MANOVA followed by the least significant difference (LSD) test.
Results: cola drinking
modified glycemia (F2,30= 6.15, p<0.006), insulinemia (F2,30= 6.59, p<0.004)
and HOMA-IR (F2,30= 8.68, p<0.001) whereas time per se affected insulinemia (F1,30=4.66,
p<0.04), HOMA-IR (F1,30=4.43, p<0.04) and BCV (F1,30=5.84, p<0.02). In
particular, regular cola drinking (C6) resulted in hyperglycemia (+15%,
p<0.07), hyperinsulinemia (+1.4 fold, p<0.03) and HOMA-IR increase (+1.8
fold, p<0.008) compared to water drinking rats (W6). After 6 months wash-out
(12 months after the beginning of the study) sustained hyperinsulinemia (+79%,
p<0.005) and HOMA-IR increase (+82%, p<0.004) were found in C12 vs W12
group. Compared to W6, BCV was decreased in W12 (-31%, p<0.05), C12 (-47%,
p<0.005) and L12 (-36%, p<0.03) and the groups showed no differences
between one another.
Conclusions: regular cola
drinking led to hyperglycemia, hyperinsulinemia, increased insulinresistance and
did not affect BCV. Differently, aging led to a decrease in BCV. A thorough interpretation
of presented evidence should consider the possibility that six months regular coladrinking
may be an insufficient time to modify beta cells viability and reduce BCV as
well.