Molecular characterization of asuppressor mutation of gigantea in Arabidopsis thaliana¨

BARBER MARIAA; JASKOLOWSKY AIME; IGLESIAS FRANCISCO

Mendoza

Congreso; XLVIII Reunión Anual de la Sociedad Argentina de Investigaciones en bioquímica y biología molecular; 2012

Sociedad Argentina de Investigaciones en bioquímica y biología molecular

Plants flower at a certain time during the year to ensure that reproductive development occurs during the favorable season. This transition is regulated by genetic and environmental factors such as temperature and photoperiod. To isolate new genes involved in the regulation of flowering time, we performed a screening to find mutants that suppress gigantea (gi) late flowering. GI is involved in photomorphogenesis, the regulation of the circadian clock and is itself regulated by temperature; so GI is an important interaction node for light and temperature signaling. We found twenty independent mutants including gis7. The aim of this work is to make a preliminary characterization and identify the gene affected in gis7. gis7 was isolated in the Columbia accession following mutagenesis with EMS. As a crossing partner for molecular mapping we used Landsberg erecta and early flowering F2 progeny was utilized for positional cloning. Plants were grown in chambers at 23ºC in LD condition (16-h-light/8-h-dark) or SD (8-h-light/16-h-dark). Total leaf number was scored in flowering time experiments. gis7 flowers early in SD but slightly early in LD at 23ºC . gis7 mapped to a locus near FLC in chromosome 5, but preliminary evidence suggests gis7 is not an allele of FLC.