CROSSTALK BETWEEN THE GLUCOCORTICOID RECEPTOR AND THE CANNABINOID RECEPTOR 1. ROLE IN THE LEARNING AND MEMORY PROCESSES

GRANJA GALEANO, G; DOMÍNGUEZ RUBIO, AP; ZAPPIA, CD; ZORRILLA ZUBILETE, MA; FITZSIMONS, CP; FRANCHI, AM; MONCZOR, F

Simposio; Third South American Symposium in Signal Tranduction and Molecular Medicine; 2015

Cannabinoid receptors (CB) are GPCRs generally coupled to Gi protein and the glucocorticoid receptor (GR) is an intracellular receptor that mediates its main functions acting as a transcription factor. CB receptor type 1 and GR are coexpressed in several areas of the central nervous system, including hippocampus, which is known to be involved in learning and memory processes. It has been suggested that the cannabinoid system participates in glucocorticoid-mediated processes regulating its activity. We have previously described that CB1 signaling regulates GR activity in neuroblastoma N2A cells in-vitro. To confirm this modulation of GR activity by CB1, we studied the in-vivo expression of Glucocorticoid Induced Leucine Zipper (GILZ), a classically GR-driven gene, in hippocampus of Wild Type and KO CB1-/- CD1 mice. In WT strain Dex produced a twofold increase in GILZ expression, and the co-treatment with CB1 antagonist AM251 was able to induce an extra increment of 60% (p