INVESTIGADORES
BUSTOS Diego Martin
congresos y reuniones científicas
Título:
14-3-3 protein family levels variations during differentiation of 3T3-L1 preadipocytes
Autor/es:
ALDANA GOJANOVICH; BUSTOS, DIEGO M; UHART, MARINA
Reunión:
Congreso; L Reunión Anual de la Sociedad Argentina de Investigaciones Bioquímicas y Biología Molecular; 2014
Resumen:
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The 14-3-3 protein family comprises
phospho-Serine/Threonine-binding proteins consisting of seven
mammalian paralogs. This protein family regulates several cellular
processes including cellular differentiation. In adipogenesis, 14-3-3
regulates the subcellular localization of TAZ, a co-repressor of PPAR
gamma dependent gene transcription. We studied the mRNA and protein
levels of the 14-3-3 family before and after differentiation of NIH
3T3-L1 preadipocytes by using qPCR and Western Blot (WB) with
specific primers and antibodies for each 14-3-3 member respectively.
The results revealed, after seven days of differentiation, a decrease
in mRNA levels of 14-3-3 beta, epsilon and zeta and an increment of
mRNA levels of 14-3-3 gamma, eta and tau, compared to
undifferentiated cells. Protein levels of each paralog were either
conserved or decreased after one week differentiation. A particularly
pronounced decrease was observed in eta isoform protein. Therefore,
an apparent discrepancy was observed between mRNA and protein levels
of 14-3-3 eta, showing a specific degradation in response to
differentiation signals in 3T3-L1 cells. Our findings indicate that
the differential expression and/or postranslational regulation of
14-3-3 family members levels during 3T3-L1 adipogenesis may be a
mechanism to indirectly modulate differentiation to the adipose
linage.