Characterization of intestinal microbiota of children and isolation of lactobacilli with probiotic potential

LORENZO PISARELLO, MJ; VINTIÑI, EO; PAGANI, F; GONZALEZ, S; MEDINA, M

Tafi del Valle

Jornada; XVIII JornadasCientificas - Asociación de Biología de Tucumán; 2011

Asociación de Biología de Tucumán

The intestinal microbiota (IM) is a complex microenvironment and includes a variety of bacterial genera and species among which the Lactobacilli are one of the main genera used as probiotics.Objective:To characterize the major bacterial genera present in the IM of eutrophic children and isolate strains of Lactobacillus for potential use as probiotics in human health. Stool of 15 healthy children were analyzed.MM:1)Count by conventional techniques: total aerobes and anaerobes,Enterobacteriaceae and lactobacilli.The N° of bacteria was determined by plate count and the results were expressed as CFU/g of feces, 2) Isolation of lactobacilli and evaluation of their morphology (GRAM stain), specific biochemical properties (API system), resistance to acid pH and bile. The results are expressed as mean total counts x g of faeces: Anaerobes: 2x109 CFU/g,Aerobes: 5x109 CFU/g, Enterobacteriaceae: 2x106 CFU /g and Lactobacillus: 3x106 CFU/g. It were isolated 2 strains of lactobacilli that with high resistance to acid pH and bile, fundamental requirements of a strain, to be defined as a probiotic. Further studies are necessary to their potential use in the development of products for preventive applications and/or particular therapeutic