B. ABORTUS INFECTION MODULATES OSTEOCYTE FUNCTION

PESCE VIGLIETTI, AYELÉN IVANA; GENTILINI, MARÍA VIRGINIA; ARRIOLA BENÍTEZ, PAULA CONSTANZA; VELÁSQUEZ, LIS NOELIA;; GUILLERMO HERNÁN GIAMBARTOLOMEI; MARÍA VICTORIA DELPINO

Congreso; LIX Reunión científica anual de la Sociedad Argentina de Investigación Clínica (SAIC). LXII Reunión Anual de la Sociedad Argentina de Inmunología (SAI).; 2014

Osteoarticular brucellosis is the most common localization ofhuman active disease. Osteocytes are the most abundant cellsof bone. They secrete factors that regulate osteoclast differentiation(cells involved in bone resorption). The aim is to determineif Brucella abortus (Ba) infection modifies osteocyte (MLO-Y4)function. Cytokine and chemokine production was determined byELISA, osteoclast differentiation was determined by microscopyas the number of multinucleated cells that express tartrate-resistantacid phosphatase (TRAP). Conexin 43, E11/gp38, integrin-α and -β,tubulin-α, CD44 expression was determined by qRT-PCR. Apoptosiswas determined by Hoechst dye 33342 (microscopy) and by AnnexinV-FITC/Propidium Iodide (PI) (cytometry). Ba infection induced thesecretion of pro-inflammatory cytokines (IL-6 and TNF-α, but notIL-1β), KC and RANKL (the main regulator of osteoclastogenesis) byosteocytes. In inflammatory condition TNF-α could be also involvedin osteoclastogenesis. Our results indicated that supernatants fromBa infected osteocytes could induce osteoclast differentiation ofmonocytes in the presence of M-CSF (p