INVESTIGADORES
VALLCANERAS Sandra
congresos y reuniones científicas
Título:
Diferente respuesta del ovario prepuberal y del primer proestro al estímulo colinérgico ganglionar
Autor/es:
DELGADO SM; CASAIS M; VALLCANERAS S; SOSA Z; RASTRILLA AM
Lugar:
Huerta Grande -Córdoba
Reunión:
Congreso; Primera Reunión Conjunta de Sociedades de Biología de República Argentina. Huerta Grande.; 2007
Resumen:
DIFFERENT RESPONSE OF PREPUBERTAL AND FIRST
PROESTROUS OVARY TO CHOLINERGIC INFLUENCES
Delgado SM, Casais M, Vallcaneras S, Sosa Z, Rastrilla AM.
Lab. Biol. de la Reproducción. Fac. Qca, Bqca. y Farmacia. Univ
Nac. de San Luis. 5700. San Luis. E-mail: sdelgado@unsl.edu.ar
The cholinergic effect on the coeliac ganglion (CG) has been described
in the prepubertal ovary of rats; yet, it remains unstudied in
the first proestrous of the estral cycle. Purpose: to elucidate the effect
of Acetylcholine in the ganglion compartment in the ex-vivo
CG-superior ovarian nerve-ovary system in rat on the ovarian release
of Progesterone (P), androstenedione (A2) and nitric oxide (NO)
in the first proestrous and to compare it with the prepubertal ovary.
The system was isolated and incubated in Krebs-Ringer-glucose
buffer in a metabolic bath in the presence of Ach (10-6 M) in CG.
Aliquots from the ovarian compartment were taken at 15, 30, 60 and
120 min. P and A2 were measured by RIA, and NO, by Griess reaction.
Statistical significance by Student´s t test at p<0.05. Results:
Ach caused a significant increase in P and A2 release at all the studied
times (p<0.05) in the first proestrous and a decrease at 30 days.
NO showed similar values in the first proestrous and at 30 days, and
the ganglion cholinergic stimulus increased liberation in both cases
(p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic
stimulus in the first proestrous favours the ovary maturation
through the increase of the hormonal secretion partly due to the low
liberation of NO in relation to the prepubertal stage.
2) and nitric oxide (NO)
in the first proestrous and to compare it with the prepubertal ovary.
The system was isolated and incubated in Krebs-Ringer-glucose
buffer in a metabolic bath in the presence of Ach (10-6 M) in CG.
Aliquots from the ovarian compartment were taken at 15, 30, 60 and
120 min. P and A2 were measured by RIA, and NO, by Griess reaction.
Statistical significance by Student´s t test at p<0.05. Results:
Ach caused a significant increase in P and A2 release at all the studied
times (p<0.05) in the first proestrous and a decrease at 30 days.
NO showed similar values in the first proestrous and at 30 days, and
the ganglion cholinergic stimulus increased liberation in both cases
(p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic
stimulus in the first proestrous favours the ovary maturation
through the increase of the hormonal secretion partly due to the low
liberation of NO in relation to the prepubertal stage.
-6 M) in CG.
Aliquots from the ovarian compartment were taken at 15, 30, 60 and
120 min. P and A2 were measured by RIA, and NO, by Griess reaction.
Statistical significance by Student´s t test at p<0.05. Results:
Ach caused a significant increase in P and A2 release at all the studied
times (p<0.05) in the first proestrous and a decrease at 30 days.
NO showed similar values in the first proestrous and at 30 days, and
the ganglion cholinergic stimulus increased liberation in both cases
(p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic
stimulus in the first proestrous favours the ovary maturation
through the increase of the hormonal secretion partly due to the low
liberation of NO in relation to the prepubertal stage.
2 were measured by RIA, and NO, by Griess reaction.
Statistical significance by Student´s t test at p<0.05. Results:
Ach caused a significant increase in P and A2 release at all the studied
times (p<0.05) in the first proestrous and a decrease at 30 days.
NO showed similar values in the first proestrous and at 30 days, and
the ganglion cholinergic stimulus increased liberation in both cases
(p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic
stimulus in the first proestrous favours the ovary maturation
through the increase of the hormonal secretion partly due to the low
liberation of NO in relation to the prepubertal stage.
t test at p<0.05. Results:
Ach caused a significant increase in P and A2 release at all the studied
times (p<0.05) in the first proestrous and a decrease at 30 days.
NO showed similar values in the first proestrous and at 30 days, and
the ganglion cholinergic stimulus increased liberation in both cases
(p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic
stimulus in the first proestrous favours the ovary maturation
through the increase of the hormonal secretion partly due to the low
liberation of NO in relation to the prepubertal stage.
2 release at all the studied
times (p<0.05) in the first proestrous and a decrease at 30 days.
NO showed similar values in the first proestrous and at 30 days, and
the ganglion cholinergic stimulus increased liberation in both cases
(p<0.01 and p<0.001, respectively). Conclusion: the ganglion cholinergic
stimulus in the first proestrous favours the ovary maturation
through the increase of the hormonal secretion partly due to the low
liberation of NO in relation to the prepubertal stage.