CIPYP   05508
CENTRO DE INVESTIGACIONES SOBRE PORFIRINAS Y PORFIRIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Haem Oxygenase gene expression is modulated by nitric oxide in soybean plants.
Autor/es:
NORIEGA, GUILLERMO; YANNARELLI, GUSTAVO; BALESTRASSE, KARINA; BATLLE, ALCIRA; TOMARO, MARíA LUJáN
Lugar:
Krakov, Polonia
Reunión:
Congreso; 5th International Congress Heme Oxygenases 2007; 2007
Resumen:
Heme oxygenase (HO, EC 1.14.99.3) catalyses the oxidative conversion of heme to
biliverdin IXa (BV) with the concomitant release of carbon monoxide and iron. Recently,
HO has been involved in the protection against oxidative stress in plants . The fact that
nitric oxide (NO), an endogenous signaling molecule in animals and plants, mediates
responses to abiotic and biotic stresses, prompted us to study, whether this molecule could
modulate HO-1 gene transcription. To fulfill this objective leaves of soybean (Glycine max
L.) plants were stimulated with Cd, employing an acute intoxication model. Cadmium
caused dehydration, chlorophyll loss and ion leakage. Semi-quantitive RT-PCR analysis
showed no augmentation of HO-1 transcript levels with respect to controls. Pretreatment
with 100 ìM sodium nitroprusiate (SNP), a well known NO donor, prevented the effects
caused by Cd. When the HO-1 mRNA levels were analyzed, a significant augmentation
(54%) was observed respect to Cd-treated plants. On the other hand, 50 or 300 ìM SNP did
not fully prevent the effects elicited by Cd. When HO-1 transcript levels were analyzed, no
significant enhancement or a down-regulation was respectively observed. The potassium
salt of 2-(4-carboxylphenyl)-4,4,5,5-tetramethylimidazoline1-oxyl-3-oxide (cPTIO), a
specific NO scavenger, arrested NO mediated protective effects against to Cd-induced
oxidative damage. These data provide an understanding of one of the possible roles that
NO can play against an oxidative insult. Nitric oxide is cytoprotective depending on its
concentration, and it was further demonstrated that this protection could be, at least in
part, mediated by an enhancement of HO-1 mRNA, as it happens with genes associated
with the antioxidant defense system.