INVESTIGADORES
MESSINA German Alejandro
congresos y reuniones científicas
Título:
Electrochemical determination of Chlortetracycline in water samples
Autor/es:
VICTORIA MEDAWAR; BERTOLINO, FRANCO A; MESSINA GERMÁN A.; PEREIRA, SIRLEY V; RABA, JULIO
Reunión:
Congreso; RICIFA; 2014
Resumen:
Tetracyclines are an important group of antibiotics
commonly used in human and veterinary medicine, aquaculture and animal
husbandry. Owing to their extensive uses, these compounds are one of the
pharmaceutical products most widely distributed as environmental pollutant. The
presence of low levels of antibiotics and their metabolites in the environment
could provide adverse effects like bacterial resistance and disruption of critical
cycles to aquatic ecology.
Therefore, it is important to develop an analysis tool
for the determination of tetracycline antibiotics in water samples. The purpose
of this work was the electrochemical determination of chlortetracycline (CTC)
in synthetic water samples.
Electrochemical experiments were performed using a BAS
100B/W electrochemical analyzer. Cyclic and square-wave voltammograms were
obtained using a three electrodes system: glassy carbon working electrode
modified with multi-walled carbon nanotubes (MWCNT), an Ag|AgCl|3M NaCl
reference electrode and a Pt wire counter electrode. For the electrode
modification 1 milligram of MWCNT was dispersed with the aid of ultrasonic
stirring for 45min in methanol/water (50:50, v/v) in an aqueous 0.1% Nafion
solution. Then, 5µL of this dispersion was dropped on the working electrode and
the solvent was evaporated under an infrared heat lamp. CTC synthetic water
samples were prepared by diluting CTC stock solution of 119 mg L-1
in purified deionized water. The CTC quantification was carried out by Square
Wave Voltammetry (SWV) in 10 mL of 0.01 M phosphate buffer.
The CTC calibration plot was performed using SWV in a
concentration range of 2 to 119 mg L-1. The calibration equation was
ΔI (μA) = 0.1026 + 0.0074 CCTC with a regression coefficient of 0.997.
Based on the results, the proposed approach was successfully used to analyze
the CTC content in water samples.