INVESTIGADORES
PIURI Mariana
congresos y reuniones científicas
Título:
Study of the interaction of cell wall polymers of Lactobacillus casei with their bacteriophages
Autor/es:
LUCÍA MALONE; MARIA EUGENIA DIETERLE; MARIANA PIURI; SANDRA M RUZAL
Lugar:
Rosario
Reunión:
Congreso; L Reunión Anual SAIB; 2014
Institución organizadora:
SAIB
Resumen:
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Understanding of
the molecular mechanisms of bacteriophage interaction with their host bacteria
can be used to develop strategies to avoid infections during dairy fermentation
processes.
We characterized the sub-cellular
fractions involved in Lactobacillus casei
phages J-1 and PL-1 recognition and adsorption.
In other lactic acid bacteria, non-proteinaceous components of the cell wall
have been described as receptors. Gram-positive bacteria surface may contain:
(i) capsular polysaccharides (CPS) like rhamnose containing glycans (RWPS)
often covalently bound to peptidoglycan (PG); (ii) wall polysaccharides (WPS)
like Lipoteichoic acid (LTA) or wall teichoic acid (WTA) that may be or not covalently
attached and (iii) extra-cellular polysaccharides (EPS) which are released into
the cell environment. Each cell wall fraction was purified by different
treatments to preserve their integrity and architecture and tested for phage
adsorption inhibition. Total Cell Wall fraction (CW) contains all components; TCA
soluble material retains WTA and/or RWPS. CW treated by boiling in 4% (w/v) SDS
remove lipoteichoic acids and proteins living purified PG. D-alanine esters and
O-acetylation were removed from CW by alkali. EPS was obtained from the culture
supernatant. TCA soluble material efficiently block
phage adsorption probably indicating the presence of phage receptors.