"Neohelice granulata from differents habitats: Alkaline phosphatases and lipase upon distinct lipidic diet?

MENDEZ, E.; LÓPEZ MAÑANES, A,A.

Buenos Aires

Encuentro; First Biology Meeting of the River Plate; 2012

Studies on digestive adjustments of N. granulata in relation to contrasting mudflat (MF) and saltmarsh (SM) are lacking. The aim was to study the effect of diets with different lipid ratio on levamisole sensitive (LS) and insensitive (LI) alkaline phosphatases (APs) and lipase (Lip) activities in hepatopancreas of individuals from MF and SM. Adult osmoconforming male crabs were fed with diets with 3.5(D1) or 5% (D2) of lipid. 10 and 30 (t30) days postingesta, APs and Lip were measured in supernatant of 10000xg15min from homogenate (0.1MTris-HCl pH 7.4). Control=values in the field (tF). APs (μmol pNPxmin-1xmgprot-1) and Lip (μmol pNPx min-1x mgprot-1) were assayed by hydrolysis of pNPP (9.5mM) (0.1M Tris-HCl,pH 8.5/4mM MgSO4 with (LI) and without 16mM Levamisole (LS=difference) or pNPpalmitate (50mM Tris/HCl, pH 8.5), respectively. Only D2 at t30 had effects compared to TF: MF= LS(50±19) was 25 fold higher. LI and Lip were similar. SM:Lip was 1.7 fold lower (71.7±7) (ANOVA, p