INVESTIGADORES
ZENI Susana Noemi
congresos y reuniones científicas
Título:
Rat mandible bone response to atherogenic diet
Autor/es:
GAMBA CA, ; MACRI V,; GONZALES CHAVES M, ; ORZUZA R, ; ZAGO V, ; RODRÍGUEZ P, ; MANDALUNIS P, ; ZENI SN, ; FRIEDMAN S
Lugar:
Buenos Aires
Reunión:
Congreso; XXVI Reunión Anual de la AAOMM; 2009
Institución organizadora:
AAOMM
Resumen:
We observed that an atherogenic diet in active growth phase did not
induce macroscopic alterations in mandible. However, diminution in
bone accretion and alterations in mandible skeletal units could lead to
structure alterations. In this study we hypothesized that atherogenic
diets could alter bone remodeling in mandible. Male weanling Wistar
rats (n=11), randomly divided into control (C) and experimental (E). C
received rodent stock diet (pellets) and E, atherogenic diet (pellets+
saturated fatty acids+cholesterol) for 7 weeks (Tf), monitoring
anthropometry and diet intake. At Tf, blood (mg/dL) lipidlipoprotein
atherogenic profile: total cholesterol (T-cho), triglycerides (TG), high
density lipoprotein-cholesterol (HDL-cho), HDL-non-cholesterol (n-
HDL-cho). Dissected hemimandibles, fixed in buffered formalinPBS
10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal
oriented sections of first mandibular molar, stained with hematoxylin
10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal
oriented sections of first mandibular molar, stained with hematoxylin
atherogenic profile: total cholesterol (T-cho), triglycerides (TG), high
density lipoprotein-cholesterol (HDL-cho), HDL-non-cholesterol (n-
HDL-cho). Dissected hemimandibles, fixed in buffered formalinPBS
10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal
oriented sections of first mandibular molar, stained with hematoxylin
10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal
oriented sections of first mandibular molar, stained with hematoxylin
lipoprotein
atherogenic profile: total cholesterol (T-cho), triglycerides (TG), high
density lipoprotein-cholesterol (HDL-cho), HDL-non-cholesterol (n-
HDL-cho). Dissected hemimandibles, fixed in buffered formalinPBS
10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal
oriented sections of first mandibular molar, stained with hematoxylin
10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal
oriented sections of first mandibular molar, stained with hematoxylin
PBS
10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal
oriented sections of first mandibular molar, stained with hematoxylin
eosin, where histomorphometric bone volume (BV) was measured.
Statistics: Student´s t test and Pearson. Results (mean±SD) at Tf: no
anthropometric or intake differences (p>0.05); In E, lipid profile
showed significant increases in T-cho (96.2±8.9 vs 56.4±2.5:
p<0.0001), n-HDL-cho (57.8±6.9 vs 21.0±3.5: p<0.0001), but the
opposite in TG (76.5± 16.4 vs 163.2±47.4: p=0.002). BV in E was
significantly reduced (35.9±6.8 vs 47.7±7.4: p=0.022). T-cho vs BV
correlation was found highly significant (p=0.0095). These results
suggest that anatherogenic diet alters bone remodeling of interradicular
bone in mandible, accounted by diminished BV. UBACyT O008.
anthropometric or intake differences (p>0.05); In E, lipid profile
showed significant increases in T-cho (96.2±8.9 vs 56.4±2.5:
p<0.0001), n-HDL-cho (57.8±6.9 vs 21.0±3.5: p<0.0001), but the
opposite in TG (76.5± 16.4 vs 163.2±47.4: p=0.002). BV in E was
significantly reduced (35.9±6.8 vs 47.7±7.4: p=0.022). T-cho vs BV
correlation was found highly significant (p=0.0095). These results
suggest that anatherogenic diet alters bone remodeling of interradicular
bone in mandible, accounted by diminished BV. UBACyT O008.
t test and Pearson. Results (mean±SD) at Tf: no
anthropometric or intake differences (p>0.05); In E, lipid profile
showed significant increases in T-cho (96.2±8.9 vs 56.4±2.5:
p<0.0001), n-HDL-cho (57.8±6.9 vs 21.0±3.5: p<0.0001), but the
opposite in TG (76.5± 16.4 vs 163.2±47.4: p=0.002). BV in E was
significantly reduced (35.9±6.8 vs 47.7±7.4: p=0.022). T-cho vs BV
correlation was found highly significant (p=0.0095). These results
suggest that anatherogenic diet alters bone remodeling of interradicular
bone in mandible, accounted by diminished BV. UBACyT O008.