Mucosal gene expression of different T helper markers is not specific for cellular rejection process in intestinal transplant patients

AGUSTINA ZAMBERNARDI; RUMBO CAROLINA; PADIN JUAN; CABANNE, ANA; SOLAR HECTOR; GONDOLESI GABRIEL; MARTIN RUMBO

Congreso; XIII International Small Bowel Transplantation Symposium; 2013

Acute cellular rejection (ACR) is a major cause of graft loss after intestinal transplant. Clinical, endoscopic and histological findings obtained upon symptoms or routine follow-up procedures are used to diagnose ACR. Improving our understanding of the process and enlarging the diagnostic tools for ACR are desirable. With this aim, we analyzed the relative expression of several genes, markers of Th1, Th2 or Th17 immune response in graft biopsies taken during endoscopic follow-up post transplant. A total of 107 samples of 18 patients (7 adults / 11 pediatrics) recipients of isolated small bowel transplant were analyzed. Among them, 48 showed normal histopathology and clinical stability, 29 correspond to rejection episodes diagnosed by histopathology and the remaining 30 correspond to different clinical or histopathological conditions (viral infections, intrabdominal collections, pancreatitis, histology inderterminate for rejection). Expression of INF-g CXCL11, CXCL10, IL-13 and IL-17 was quantified by RT-qPCR, using of b-actin as normalizer and using the group of normal samples as reference. Different clinical conditions such as infectious enteritis may influence the levels of expression of immune related genes and there is still missing a specific marker of rejection at the gene expression level. During rejection, a correlation between lamina propria cellularity and IFNg and IL13 expression was determined. The analysis of a larger number of samples and incorporation of new genes will improve the characterization and help to the differential diagnosis of ACR vs infectious events that require opposed therapeutic strategies. .