DEVELOPMENT AND VALIDATION OF A MICROMETHOD FOR THE QUANTIFICATION OF 5-ALKYLRESORCINOLS IN CEREAL GRAINS AND DERIVED PRODUCTS

SAMPIETRO, D.A.; BELIZAN, M.M.E.; VATTUONE, M.A.; CATALAN, C.A.N.

Tucuman

Congreso; XXX ANNUAL SCIENTIFIC MEETING TUCUMAN BIOLOGY ASSOCIATION; 2013

TUCUMAN BIOLOGY ASSOCIATION

The aim of this work was the development and validation of a colorimetric micromethod for the quantification of 5nARs based on the use of a Fast Blue RR salt. The microcolorimetric technique was optimized in 96-well microplates using several 5nAR standards, solvents, different types and concentrations of basifying reagents and several concentrations of Fast Blue RR. Absorbance was determined at different times and wavelengths. The optimized technique was used for the quantification of 5nARs in samples of wheat and rye grains and derived food products. Results were validated by a Bland-Altman analysis which included the contents of 5nARs determined by the use of a Fast Blue B salt. The highest sensitivity for 5nARs was obtained at 490 nm with 0.025% ethanolic Fast Blue RR and 5% K2CO3. The correlation between the 5nAR contents generated using the two colorimetric methods was very high (R2 = 0.9944) with a small positive bias near zero (R2=0.0401). In the new micromethod, incubation time was reduced to 15 minutes and the reaction products were stable for 1 h.These advantages offer fast analysis of 5nARs in cereal grains and their derived products, with low consumption of reagents and solvents.