INGEBI   02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Towards synthetic disease resistance genes
Autor/es:
GIANNAKOPOULOU, ARTEMIS; PAIS, MARINA; CHAPARRO GARCÍA, ANGELA; SEGRETIN, MARÍA EUGENIA; KAMOUN, SOPHIEN
Lugar:
Rodas
Reunión:
Congreso; XVI International Congress on Molecular Plant-Microbe Interactions; 2014
Institución organizadora:
IS-MPMI
Resumen:
Plants and pathogens are engaged in a co-evolutionary arms race, in which plants deploy
various defense mechanisms and pathogens develop ways to modulate host processes and
immunity. An important component of the plant defense machinery involves intracellular
immunoreceptors of the nucleotide-binding leucine-rich repeat-containing protein family (NBLRR).
NB-LRRs typically recognize pathogen effector proteins with avirulent activities, and
activate a response known as NB-LRR-triggered immunity (NTI). R3a and I2 are orthologous
NB-LRRs from potato and tomato that recognize effectors of the late blight oomycete
pathogen Phytophthora infestans and the wilt causing ascomycete Fusarium oxysporum f. sp.
lycopersici (FOL) respectively. However, particular races of these pathogens have evolved
stealthy effectors that evade recognition by R3a and I2. Our goal is to create synthetic NBLRR
mutants with expanded pathogen recognition specificities to develop broad-spectrum
solutions to important plant pathogens. In a previous study we identified 8 single-residue
mutations in the R3a protein that conferred expanded response to Phytophthora sp. effectors.
We investigated whether these mutations alter the response profile of I2, and recovered I2
mutants with expanded spectrum of effector response from diverse pathogens. We are
currently evaluating whether this expanded response correlates with a broader resistance
spectrum using both transient and stable transgenic systems. These results could lead to new
insights into the molecular interactions underlying pathogen perception by plants, building up
our knowledge on basic and applied plant pathology.