Regularion of the Expression of Genes in Plants by Photoreceptors

RODRÍGUEZ BATILLER MJ, TREJO EC, STANELONI RJ.

Iguazú, Misiones

Congreso; XL Annual Meeting ARGENTINE SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY; 2004

Sociedad Argentina de Investigación Bioquímica y Biología Molecular (SAIB)

Light is one of the most important factors in plant development of plants. Plants have involved systems that allow them to sense the light quality and intensity. Phytochromes, specific photoreceptors, are in charge of sensing the luminous signal, which is change inthe gene expression followed by.We study the signal transduction chains of the plant photoreceptors and the transcription factors that act at the end of the signaling cascades. With this purpose, the full-length promoter Lhcb1*2and several DNA fragments of it were fused to a gusA coding region (GUS), and transferred to Arabidopsis thaliana plants via Agrobacterium tumefaciens. Transgenics plants were treated underdifferent light conditions and the GUS activity was considered a measure of the Lhcb1*2 gene expression.We found that a 35 bp fragment of the tobacco Lhcb1*2 promoter was involved in the phyA mediated high-irradiance response (HIR). The 35 bp fragment was used in one-hybrid experiments to isolate the transcription factor involved in the HIR response. Reverse genetic was used to address the "in vivo" role of the cloned transcription factor.